NDLI: Molecular evidence of outcrossing rate variability in Brassica napus

Content Provider	Springer Nature Link
Author	Soengas, P. Padilla, G. Francisco, M. Velasco, P. Cartea, M. E.
Copyright Year	2011
Abstract	A leafy crop of Brassica napus L. called nabicol has been grown by farmers in northwestern Spain for many years, being an important horticultural product during the winter season. A collection of landraces of a Brassica napus leafy crop called nabicol is kept at ‘Misión Biológica de Galicia’ (CSIC-Spain) which can be used to search for desirable characteristics or to produce new commercial varieties to release in the market. The assessment of the mating system of nabicol landraces is particularly important to carry on adequate breeding and genetic conservation programs. The objective of this work is to estimate the outcrossing rate in nabicol under controlled pollinator conditions using SSRs. Pairs of flowering plants taken from nabicol landrace MBG-BRS0039 were placed in separated isolation cages and bumble bees (Bombus sp.) were released for facilitating the crosses between plants. A seed sample from each plant in the cross was analyzed by SSRs that were polymorphic in the parental population. We found that the crop is partially allogamous and that there is genetic variation for the outcrossing rate among individuals. Several consequences for the maintenance and genetic improvement of the crop are discussed. The existence of genetic variability for this trait is a valuable tool that will allow us to study the genetic mechanisms underlying the mating system of this crop.
Starting Page	301
Ending Page	306
Page Count	6
File Format	PDF
ISSN	00142336
Journal	Euphytica
Volume Number	180
Issue Number	3
e-ISSN	15735060
Language	English
Publisher	Springer Netherlands
Publisher Date	2011-01-30
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Brassica napus Nabicol landraces Mating system Outcrossing rate Microsatellites SSRs Plant Pathology Plant Genetics & Genomics Plant Sciences Plant Physiology Biotechnology
Content Type	Text
Resource Type	Article
Subject	Genetics Plant Science Horticulture Agronomy and Crop Science

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