NDLI: Identification of ‘Sib’ plants in hybrid cauliflowers using microsatellite markers

Content Provider	Springer Nature Link
Author	Astarini, Ida A. Plummer, Julie A. Lancaster, Rachel A. Yan, Guijun
Copyright Year	2008
Abstract	Hybrid cauliflowers have been developed to exploit heterosis and to improve uniformity of production. Two breeding systems are commonly employed, self-incompatibility (SI) and cytoplasmic male sterility (CMS). Sibs, assumed to be self-inbred, often contaminate hybrid seed lots in the SI system and whilst self-inbreeding is not possible in the CMS system, plants that look like sibs occur. The objective of this study was to develop microsatellite markers for male and female cauliflower parent lines of both SI and CMS systems and to use them to screen sibs and aberrant plants in F1 hybrids. Fifty six pairs of microsatellite primers were screened and 8 primer pairs produced co-dominant markers in parent plants and two pairs of markers were chosen for purity testing of F1 hybrid seeds. Controlled pollinations were conducted in the glasshouse to produce hybrid and selfed-seeds. These seeds were grown in a field trial to identify morphologically normal and sib plants and to assess the reliability of microsatellite markers in detecting sib plants. Microsatellite analysis of morphological sib plants from the SI system revealed that these were not always self-inbred, in contrast, most self-inbred plants showed normal growth. Similarly, all morphological sibs from the CMS system showed hybrid bands. This suggests that morphological sibs were not always due to selfing but possibly to an interaction between genetic and environmental factors and this requires further investigation.
Starting Page	309
Ending Page	316
Page Count	8
File Format	PDF
ISSN	00142336
Journal	Euphytica
Volume Number	164
Issue Number	2
e-ISSN	15735060
Language	English
Publisher	Springer Netherlands
Publisher Date	2008-02-03
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Brassica oleracea var. botrytis Cytoplasmic male sterility Self-inbred Self-incompatibility SSR Biotechnology Plant Physiology Plant Pathology Plant Genetics & Genomics Plant Sciences
Content Type	Text
Resource Type	Article
Subject	Genetics Plant Science Horticulture Agronomy and Crop Science

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Cloning and Transcript Analyses of the Chimeric Gene Associated With Cytoplasmic Male Sterility in Cauliflower (Brassica oleracea var. botrytis)

Cytoplasmic male sterility with self-incompatibility, a novel approach to utilizing heterosis in rapeseed (Brassica napus L.)

Cloning and transcript analyses of the chimeric gene associated with cytoplasmic male sterility in cauliflower (Brassica oleracea var. botrytis)

Studies on heterosis and combining ability in early cauliflower (Brassica oleracea var. botrytis L.)

Chloroplast and mitochondrial SSR help to distinguish allo-cytoplasmic male sterile types in cabbage (Brassica oleracea L. var. capitata)

Superior CMS (Ogura) lines with better combining ability improve yield and maturity in cauliflower (Brassica oleracea var. botrytis)

Efficient doubled haploid production in microspore culture of loose-curd cauliflower (Brassica oleracea var. botrytis)

Distribution of S-haplotypes and its relationship with self-incompatibility in Brassica oleracea. Part 1. In inbred lines of cauliflower (B. oleracea var ‘botrytis’)

“Aberrant” plants in cauliflower: 1. Phenotype and heredity

Identification of ‘Sib’ plants in hybrid cauliflowers using microsatellite markers

Similar Documents

Cloning and Transcript Analyses of the Chimeric Gene Associated With Cytoplasmic Male Sterility in Cauliflower (Brassica oleracea var. botrytis)

Cytoplasmic male sterility with self-incompatibility, a novel approach to utilizing heterosis in rapeseed (Brassica napus L.)

Cloning and transcript analyses of the chimeric gene associated with cytoplasmic male sterility in cauliflower (Brassica oleracea var. botrytis)

Studies on heterosis and combining ability in early cauliflower (Brassica oleracea var. botrytis L.)

Chloroplast and mitochondrial SSR help to distinguish allo-cytoplasmic male sterile types in cabbage (Brassica oleracea L. var. capitata)

Superior CMS (Ogura) lines with better combining ability improve yield and maturity in cauliflower (Brassica oleracea var. botrytis)

Efficient doubled haploid production in microspore culture of loose-curd cauliflower (Brassica oleracea var. botrytis)

Distribution of S-haplotypes and its relationship with self-incompatibility in Brassica oleracea. Part 1. In inbred lines of cauliflower (B. oleracea var ‘botrytis’)

“Aberrant” plants in cauliflower: 1. Phenotype and heredity

Identification of ‘Sib’ plants in hybrid cauliflowers using microsatellite markers