NDLI: Induction of Fusarium wilt (Fusarium oxysporum f. sp. pisi) resistance in garden pea using induced mutagenesis and in vitro selection techniques

Content Provider	Springer Nature Link
Author	Sharma, Akhilesh Rathour, Rajeev Plaha, P. Katoch, Viveka Khalsa, G. S. Patial, Vandana Singh, Yudhvir Pathania, N. K.
Copyright Year	2009
Abstract	Wilt caused by Fusarium oxysporum f. sp. pisi is a serious production constraint for peas worldwide. An attempt was made to isolate wilt-resistant mutants in two susceptible pea genotypes, Arkel and Azad P-1, employing induced mutagenesis and in vitro selection techniques. Two thousand seeds of each genotype were mutagenized either with ethyl methane sulfonate (EMS, 0.2% and 0.3%) or gamma rays (5-22.5 kR) in 60Co gamma cell for three consecutive years. Screening of different mutagenized populations under wilt-sick plots resulted in the isolation of 25 mutants exhibiting complete or enhanced wilt resistance compared to parental genotypes. Five of these wilt-resistant mutants also outperformed the susceptible background genotypes in terms of yield and other horticultural traits. Efforts were also made to isolate wilt-resistant regenerants from callus cultures exhibiting insensitivity to culture filtrate (CF) of F. oxysporum f. sp. pisi. A total of 250 regenerants (R 0) were obtained from CF-insensitive calli selected from medium supplemented with 20% culture filtrate. When evaluated in artificially inoculated sick plots, only five R 2 lines obtained from the regenerants exhibited enhanced wilt resistance compared to parental cultivars. However, the selected lines did not exhibit resistance levels equivalent to those shown by wilt-resistant lines isolated through in vivo mutagenesis. To conclude, induced mutagenesis through irradiation and EMS treatments exhibited superiority over in vitro selection for inducing wilt resistance in peas.
Starting Page	345
Ending Page	356
Page Count	12
File Format	PDF
ISSN	00142336
Journal	Euphytica
Volume Number	173
Issue Number	3
e-ISSN	15735060
Language	English
Publisher	Springer Netherlands
Publisher Date	2009-12-31
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Culture filtrate EMS Fusarium oxysporum f. sp. pisi Irradiation Mutation Pisum sativum L. Resistance Biotechnology Plant Physiology Plant Pathology Plant Genetics & Genomics Plant Sciences
Content Type	Text
Resource Type	Article
Subject	Genetics Plant Science Horticulture Agronomy and Crop Science

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