NDLI: Marker-assisted breeding for rf1, a nuclear gene controlling A1 CMS in sorghum (Sorghum bicolor L. Moench)

Content Provider	Springer Nature Link
Author	Gao, Jianming Xia, Buxian Luo, Feng Sun, Shoujun Pei, Zhongyou Gui, Zhi Yuan, Qinghua Li, Xianglin
Copyright Year	2013
Abstract	A study on marker-assisted selection (MAS) for the rf1 gene, which controls pollen sterility in the sorghum A1 cytoplasm, was conducted on the offspring population of two crosses between a maintainer line, BTx-622, and two sweet sorghum lines, BJ-299 and Lunen-2, to test the effectiveness of the MAS method and develop maintainer lines with sweet and juicy stalks and corresponding cytoplasmic-nuclear male sterility (CMS) lines. The simple sequence repeat marker Xtxp18 exhibited a high accuracy (95.098 %) for selecting recessive homozygotes for the rf1 gene. The segregation ratio matched the expected ratio calculated according to the reported genetic distance in the F2 population of the two crosses used. Finally, four excellent maintainer lines/CMS line pairs (F5/BC3) with high stalk juice and stalk juice sugar contents were developed. The MAS method based on Xtxp18 for the sorghum rf1 gene could be used for hybrid breeding programs at a low cost in the future.
Starting Page	383
Ending Page	390
Page Count	8
File Format	PDF
ISSN	00142336
Journal	Euphytica
Volume Number	193
Issue Number	3
e-ISSN	15735060
Language	English
Publisher	Springer Netherlands
Publisher Date	2013-05-28
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Sorghum Cytoplasmic-nuclear male sterility rf1 gene Marker-assisted selection Stalk juice sugar content Stalk juice content Plant Sciences Plant Genetics & Genomics Plant Pathology Plant Physiology Biotechnology
Content Type	Text
Resource Type	Article
Subject	Genetics Plant Science Horticulture Agronomy and Crop Science

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