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| Content Provider | Springer Nature Link |
|---|---|
| Author | Brunings, Asha M. Moyer, Catalina Peres, Natalia Folta, Kevin M. |
| Copyright Year | 2010 |
| Abstract | Simple sequence repeats (SSRs or microsatellites) are frequently used as a robust, rapid, and relatively inexpensive means of genotyping. Recent reports in cultivated strawberry (Fragaria × ananassa) have identified a widely applicable set of SSRs that permits discrimination of closely-related genotypes. In the present study this same set of SSRs is analyzed in cultivars commonly grown in the state of Florida, as well as advanced breeding selections from the University of Florida program. Nine primer pairs have been used to produce discrete SSR patterns for all lines tested, including diagnostic sets that distinguish between closely-related cultivars and/or breeding selections. A comparison of markers to known pedigrees is presented, along with analysis of relative relatedness between lines studied. We also detail important technical considerations for transferability and limitations of these technologies between laboratories. The resulting genotype data for cultivars commonly used in Florida are accessible as supplemental data in graphic format and allow comparison with other cultivars. Users can thus select the most diagnostic SSRs to test the lines in question. These resources provide new tools for breeders and nurseries to authenticate genotypes, follow inheritance patterns and enforce patent protection. As important, this report underscores the strengths and weaknesses of applying the original methodologies to a different plant population in independent laboratories. |
| Starting Page | 63 |
| Ending Page | 75 |
| Page Count | 13 |
| File Format | |
| ISSN | 00142336 |
| Journal | Euphytica |
| Volume Number | 173 |
| Issue Number | 1 |
| e-ISSN | 15735060 |
| Language | English |
| Publisher | Springer Netherlands |
| Publisher Date | 2010-01-12 |
| Publisher Place | Dordrecht |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Microsatellites SSR markers Fragaria Strawberry Genotyping Fingerprinting Biotechnology Plant Physiology Plant Pathology Plant Genetics & Genomics Plant Sciences |
| Content Type | Text |
| Resource Type | Article |
| Subject | Genetics Plant Science Horticulture Agronomy and Crop Science |
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