NDLI: A physical map of chromosome 4Hch from H. chilense containing SSR, STS and EST-SSR molecular markers

Content Provider	Springer Nature Link
Author	Said, Mahmoud Cabrera, Adoración
Copyright Year	2009
Abstract	The construction of a physical map of chromosome 4Hch from Hordeum chilense containing molecular markers capable of detecting segments of this chromosome in a wheat background would be very useful for marker-assisted introgression of 4Hch chromatin into both durum and common wheat. With this aim, the applicability of 106 barley chromosome 4H primers (62 SSRs and 44 STSs) to amplify markers showing polymorphism between H. chilense and both common or bread and durum wheat was investigated. Twenty-five SSR (40.3%) and six STS (13.6%) barley primer pairs consistently amplified H. chilense products. Eight SSR (12.9%) and four STS (9.1%) barley primers were polymorphic between H. chilense and both common and durum wheat, 10 of them (6 SSRs and 4 STSs) were located on chromosome 4Hch using both the addition line of chromosome 4Hch in Chinese Spring wheat and a tritordeum line (an amphiploid between H. chilense and T. turgidum) nullisomic for chromosome 4Hch. Additionally, 18 EST-SSR barley markers previously located on chromosome 4Hch were screened for polymorphism; 15 were polymorphic between H. chilense and both durum and common wheat. For physical mapping we used a ditelosomic tritordeum line for the short arm of chromosome 4Hch and a tritordeum line homozygous for a 70% terminal deletion of the long arm of 4Hch. A total of 25 markers (6 SSRs, 4 STSs and 15 EST-SSRs) were mapped to chromosome 4Hch. Eight markers were allocated on the 4HchS, eight were mapped in the 30% proximal region of 4HchL and nine were on the 70% distal region of 4HchL, respectively. Arm location on barley chromosome 4H was also carried out using both 4HS and 4HL ditelosomic addition lines in wheat. All markers mapped may have a role in marker-assisted introgression of chromatin segments of chromosome 4Hch in both durum and common wheat backgrounds.
Starting Page	253
Ending Page	259
Page Count	7
File Format	PDF
ISSN	00142336
Journal	Euphytica
Volume Number	167
Issue Number	2
e-ISSN	15735060
Language	English
Publisher	Springer Netherlands
Publisher Date	2009-01-30
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Barley Chromosome 4H Introgression Physical mapping Biotechnology Plant Physiology Plant Pathology Plant Genetics & Genomics Plant Sciences
Content Type	Text
Resource Type	Article
Subject	Genetics Plant Science Horticulture Agronomy and Crop Science

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4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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