NDLI: Identifying best methods for routine ELISA detection of microcystin in seafood

Content Provider	Springer Nature Link
Author	Preece, Ellen P. Moore, Barry C. Swanson, Mark E. Hardy, F. Joan
Copyright Year	2015
Abstract	Ingestion of water contaminated with the cyanotoxin, microcystin (MC), can pose serious health risks to humans. MC is also known to accumulate in seafood; however, this exposure pathway is much less understood. A fundamental element of this uncertainty is related to analytical difficulties. Commercially available enzyme-linked immunosorbent assays (ELISAs) offer one of the best options for routine MC detection, but methods of detecting MC in tissue are far from standardized. We spiked freshwater finfish and marine mussel tissues with MC, then compared recovery rates using four different preparation protocols and two ELISA types (polyclonal anti-MC-ADDA/direct monoclonal (DM)). Preparation protocol, type of ELISA, and seafood tissue variety significantly affected MC detection. This is the first known study to use DM ELISA for tissue analyses, and our findings demonstrate that DM ELISA combined with a short solvent extraction results in fewer false positives than other commonly used methods. This method can be used for rapid and reliable MC detection in seafood.
Starting Page	1
Ending Page	10
Page Count	10
File Format	PDF
ISSN	01676369
Journal	Environmental Monitoring and Assessment
Volume Number	187
Issue Number	2
e-ISSN	15732959
Language	English
Publisher	Springer International Publishing
Publisher Date	2015-01-27
Publisher Place	Cham
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Cyanobacteria ELISA Microcystin Seafood Method comparison Environmental Monitoring/Analysis Environmental Management Ecotoxicology Atmospheric Protection/Air Quality Control/Air Pollution Ecology
Content Type	Text
Resource Type	Article
Subject	Pollution Medicine Management, Monitoring, Policy and Law

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