NDLI: DNA integrity of chub erythrocytes (Squalius cephalus L.) as an indicator of pollution-related genotoxicity in the River Sava

Content Provider	Springer Nature Link
Author	Pavlica, Mirjana Štambuk, Anamaria Malović, Lana Mladinić, Marin Klobučar, Göran I. V.
Copyright Year	2010
Abstract	An alkaline comet assay and a micronucleus test were carried out on erythrocytes of the European chub, Squalius cephalus L., collected in spring and autumn in 2005 and 2006 at three sampling sites in River Sava, near Zagreb, Croatia. The results of comet assay showed the lowest genotoxic influence at the least polluted site, while higher DNA damage was observed at the polluted sites. Although the basal levels of DNA damage were elevated, a clear gradation of DNA damage was found due to pollution intensity in all sampling periods. The lowest cytogenetic damage as revealed by the micronucleus test (MNT) was observed as well at the least polluted site. High variations in MN frequency were observed between sampling periods, although the number of micronucleated erythrocytes was consistently the highest one at the polluted site. The comet assay as a biomarker of genotoxic effect exhibited higher sensitivity in discriminating the genotoxic capacity of studied polluted sites while the MNT was less sensitive. However, both tests should be used together in biomonitoring studies because they can reveal different aspects of DNA damage; comet assay, the early event of genotoxic exposure, and MNT, its final result as a mutagenic potential.
Starting Page	85
Ending Page	94
Page Count	10
File Format	PDF
ISSN	01676369
Journal	Environmental Monitoring and Assessment
Volume Number	177
Issue Number	1-4
e-ISSN	15732959
Language	English
Publisher	Springer Netherlands
Publisher Date	2010-07-27
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Comet assay Micronucleus test Chub Genotoxicity Biomonitoring Atmospheric Protection/Air Quality Control/Air Pollution Environmental Management Ecotoxicology Ecology Environmental Monitoring/Analysis
Content Type	Text
Resource Type	Article
Subject	Pollution Medicine Management, Monitoring, Policy and Law

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