NDLI: Cloning metallothionein gene in Zacco platypus and its potential as an exposure biomarker against cadmium

Content Provider	Springer Nature Link
Author	Lee, Sangwoo Kim, Cheolmin Kim, Jungkon Kim, Woo Keun Shin, Hyun Suk Lim, Eun Suk Lee, Jin Wuk Kim, Sunmi Kim, Ki Tae Lee, Sung Kyu Choi, Cheol Young Choi, Kyungho
Copyright Year	2015
Abstract	Zacco platypus, pale chub, is an indigenous freshwater fish of East Asia including Korea and has many useful characteristics as indicator species for water pollution. While utility of Z. platypus as an experimental species has been recognized, genetic-level information is very limited and warrants extensive research. Metallothionein (MT) is widely used and well-known biomarker for heavy metal exposure in many experimental species. In the present study, we cloned MT in Z. platypus and evaluated its utility as a biomarker for metal exposure. For this purpose, we sequenced complete complementary DNA (cDNA) of MT in Z. platypus and carried out phylogenetic analysis with its sequences. The transcription-level responses of MT gene following the exposure to CdCl2 were also assessed to validate the utility of this gene as an exposure biomarker. Analysis of cDNA sequence of MT gene demonstrated high conformity with those of other fish. MT messenger RNA (mRNA) expression and enzymatic MT content significantly increased following CdCl2 exposure in a concentration-dependent manner. The level of CdCl2 that resulted in significant MT changes in Z. platypus was within the range that was reported from other fish. The MT gene of Z. platypus sequenced in the present study can be used as a useful biomarker for heavy metal exposure in the aquatic environment of Korea and other countries where this freshwater fish species represents the ecosystem.
Starting Page	1
Ending Page	7
Page Count	7
File Format	PDF
ISSN	01676369
Journal	Environmental Monitoring and Assessment
Volume Number	187
Issue Number	7
e-ISSN	15732959
Language	English
Publisher	Springer International Publishing
Publisher Date	2015-06-20
Publisher Place	Cham
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Zacco platypus Methallothionein Cadmium Surface water Biomarker Molecular cloning Environmental Monitoring/Analysis Environmental Management Ecotoxicology Atmospheric Protection/Air Quality Control/Air Pollution Ecology
Content Type	Text
Resource Type	Article
Subject	Pollution Medicine Management, Monitoring, Policy and Law

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