NDLI: Transformation of fungal isolates with avirulence genes provides tools for identification of corresponding resistance genes in the host plant

Content Provider	Springer Nature Link
Author	Van de Wouw, Angela P. Elliott, Candace E. Howlett, Barbara J.
Copyright Year	2014
Abstract	The identification and characterisation of resistance genes in breeding lines and commercial cultivars is often required for the development of disease control strategies such as rotation, stacking or spatial deployment of resistance genes. Sets of differential fungal isolates can be used to identify resistance genes present in breeding lines and cultivars, however, interpretation of results can be hindered by the presence of novel (previously uncharacterised) resistance genes or the stacking of multiple resistance genes. In this study, we generate a set of Leptosphaeria maculans differential addition isolates by transforming isolates with single avirulence genes (AvrLm1 or AvrLm4-7). These transformants have been screened against cultivars with unidentified resistance genes to confirm the presence of the corresponding resistance genes. This is a novel approach of identifying resistance genes on the basis of the response of host plants to inoculation with fungal isolates transformed with the corresponding avirulence genes.
Starting Page	875
Ending Page	882
Page Count	8
File Format	PDF
ISSN	09291873
Journal	European Journal of Plant Pathology
Volume Number	140
Issue Number	4
e-ISSN	15738469
Language	English
Publisher	Springer Netherlands
Publisher Date	2014-08-08
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Brassica napus Leptosphaeria maculans Avirulence genes Disease resistance genes Plant Pathology Plant Sciences Ecology Agriculture Life Sciences
Content Type	Text
Resource Type	Article
Subject	Plant Science Horticulture Agronomy and Crop Science

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