NDLI: MAT gene idiomorphs suggest a heterothallic sexual cycle in the citrus pathogen Phyllosticta citricarpa

Content Provider	Springer Nature Link
Author	Amorim, Renata Savi, Daiani Cristina Ferreira Maba, Lisandra Aluizio, Rodrigo Goulin, Eduardo Henrique Takita, Marco Aurélio Machado, Marcos Antonio Glienke, Chirlei
Copyright Year	2016
Abstract	Sexual reproduction in fungi is controlled by mating type genes, which are located at the MAT locus. In this study, we investigated the structure of this locus in the phytopathogenic fungus Phyllosticta citricarpa, the causal agent of citrus black spot disease. Despite intensive study, its sexual state has never been observed in single-spore culture. Through analysis of the genome sequences of two individual P. citricarpa isolates, the sequence of the DNA lyase gene was identified and, as previously reported in the literature, the mating type genes were located in the 3′ flanking region of this gene. The results suggested that P. citricarpa is heterothallic, owing to the exclusive presence of the MAT1–1 or MAT1–2 gene in individual strains. In order to characterize the MAT locus, we designed primers to amplify this region. P. citricarpa was found to have complete and apparently functional copies of MAT genes, containing α-1 and HMG domains, present in different isolates. In addition to MAT1–2-1 and MAT1–1-1 genes, the MAT1–1-4 gene was located in the 5′ flanking region of the MAT1–1-1 gene and the MAT1–2-5 gene was located in 5′ flanking region of the MAT1–2-1 gene. A multiplex PCR protocol was also developed to differentiate P. citricarpa idiomorphs, which can be used in distribution and incidence studies of mating type strains, in order to determine the occurrence of sexual reproduction and to facility crossing studies. Furthermore, in Brazil, the two idiomorphs occur in a 1:1 ratio, which is expected in sexually reproducing populations.
Starting Page	325
Ending Page	337
Page Count	13
File Format	PDF
ISSN	09291873
Journal	European Journal of Plant Pathology
Volume Number	147
Issue Number	2
e-ISSN	15738469
Language	English
Publisher	Springer Netherlands
Publisher Date	2016-07-26
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Phyllosticta citricarpa Citrus black spot Sexual reproduction Mating type genes Plant Pathology Plant Sciences Ecology Agriculture Life Sciences
Content Type	Text
Resource Type	Article
Subject	Plant Science Horticulture Agronomy and Crop Science

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Histopathology of black spot symptoms in sweet oranges

A new method for inoculation of fruit with Guignardia citricarpa, the causal agent of citrus black spot

Potential distribution of citrus black spot in the United States based on climatic conditions

Spatial pattern of black spot incidence within citrus trees related to disease severity and pathogen dispersal

Climatic distribution of citrus black spot caused by Phyllosticta citricarpa. A historical analysis of disease spread in South Africa

Pathogenicity, colony morphology and diversity of isolates of Guignardia citricarpa and G. mangiferae isolated from Citrus spp.

Response to the letter on “Climatic distribution of citrus black spot caused by Phyllosticta citricarpa. A historical analysis of disease spread in South Africa” by Fourie et al. (2017)

A loop-mediated isothermal amplification-based method for confirmation of Guignardia citricarpa in citrus black spot lesions

Crop loss, aetiology, and epidemiology of citrus black spot in Ghana

MAT gene idiomorphs suggest a heterothallic sexual cycle in the citrus pathogen Phyllosticta citricarpa

Similar Documents

Histopathology of black spot symptoms in sweet oranges

A new method for inoculation of fruit with Guignardia citricarpa, the causal agent of citrus black spot

Potential distribution of citrus black spot in the United States based on climatic conditions

Spatial pattern of black spot incidence within citrus trees related to disease severity and pathogen dispersal

Climatic distribution of citrus black spot caused by Phyllosticta citricarpa. A historical analysis of disease spread in South Africa

Pathogenicity, colony morphology and diversity of isolates of Guignardia citricarpa and G. mangiferae isolated from Citrus spp.

Response to the letter on “Climatic distribution of citrus black spot caused by Phyllosticta citricarpa. A historical analysis of disease spread in South Africa” by Fourie et al. (2017)

A loop-mediated isothermal amplification-based method for confirmation of Guignardia citricarpa in citrus black spot lesions

Crop loss, aetiology, and epidemiology of citrus black spot in Ghana

MAT gene idiomorphs suggest a heterothallic sexual cycle in the citrus pathogen Phyllosticta citricarpa