NDLI: Development of a multiplex PCR diagnostic assay for the detection of Stagonosporopsis species associated with ray blight of Asteraceae

Content Provider	Springer Nature Link
Author	Vaghefi, Niloofar Hay, Frank S. Pethybridge, Sarah J. Ford, Rebecca Taylor, Paul W. J.
Copyright Year	2016
Abstract	Ray blight, a destructive disease of Asteraceae worldwide, is caused by three morphologically similar but phylogenetically distinct species; Stagonosporopsis chrysanthemi, S. inoxydabilis and S. tanaceti. Stagonosporopsis chrysanthemi has been reported as a specific pathogen of chrysanthemum while S. inoxydabilis has been found associated with various Asteraceae. Stagonosporopsis tanaceti has only been reported in Australia, causing substantial crop loss on pyrethrum. All three species were shown to infect and cause disease on in vitro grown pyrethrum plants, hence, S. chrysanthemi and S. inoxydabilis may pose a significant biosecurity threat to the Australian pyrethrum industry. All these Stagonosporopsis species are also Level 2 quarantine pathogens in Europe. Rapid and accurate detection and differentiation of these species is a priority for ray blight management in Australia and in Europe. Accordingly, three species-specific PCR-based assays, targeted to the intergenic spacer of the nuclear ribosomal DNA, were developed. The specificity of each assay was confirmed against 21 Stagonosporopsis spp. as well as 14 pathogenic and saprophytic fungal species commonly found in association with pyrethrum in Australia. The primers were highly sensitive and specific to the target species, detecting down to 4 fg of genomic DNA. These primers were further used in a multiplex PCR to differentiate the presence of the three Stagonosporopsis spp. based on variable sized amplicons in a single reaction.
Starting Page	581
Ending Page	595
Page Count	15
File Format	PDF
ISSN	09291873
Journal	European Journal of Plant Pathology
Volume Number	146
Issue Number	3
e-ISSN	15738469
Language	English
Publisher	Springer Netherlands
Publisher Date	2016-04-28
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Chrysanthemi Inoxydabilis Tanaceti Intergenic spacer Ribosomal DNA Pyrethrum Chrysanthemum Plant Pathology Plant Sciences Ecology Agriculture Life Sciences
Content Type	Text
Resource Type	Article
Subject	Plant Science Horticulture Agronomy and Crop Science

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Revisiting Stagonosporopsis species associated with chrysanthemum and pyrethrum ray blight

Stagonosporopsis spp. associated with ray blight disease of Asteraceae

Disease cycle of Stagonosporopsis tanaceti in pyrethrum plants

Histopathology of S. tanaceti infection in pyrethrum leaf lamina

Rapid Changes in the Genetic Composition of Stagonosporopsis tanaceti Population in Australian Pyrethrum Fields.

Sequence Analysis and Detection of Ralstonia solanacearum by Multiplex PCR Amplification of 16S–23S Ribosomal Intergenic Spacer Region with Internal Positive Control

Two Stagonosporopsis species identified as causal agents of gummy stem blight epidemics of gherkin cucumber (Cucumis sativus) in Karnataka, India

Characterization of mating type genes supports the hypothesis that Stagonosporopsis chrysanthemi is homothallic and provides evidence that Stagonosporopsis tanaceti is heterothallic

Erratum to: Two Stagonosporopsis species identified as causal agents of gummy stem blight epidemics of gherkin cucumber (Cucumis sativus) in Karnataka, India

Development of a multiplex PCR diagnostic assay for the detection of Stagonosporopsis species associated with ray blight of Asteraceae

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Development of a multiplex PCR diagnostic assay for the detection of Stagonosporopsis species associated with ray blight of Asteraceae