NDLI: An assessment of the potential of the microbial assay for risk assessment (MARA) for ecotoxicological testing

Content Provider	Springer Nature Link
Author	Fai, Patricia Bi Grant, Alastair
Copyright Year	2010
Abstract	Rapid microscale toxicity tests make it possible to screen large numbers of compounds and greatly simplify toxicity identification evaluation and other effect directed chemical analyses of effluents or environmental samples. Tests using Vibrio fischeri (such as Microtox®) detect toxicants that cause non-specific narcosis, but are insensitive to other important classes of contaminants. The microbial assay for risk assessment (MARA) is a 24 h multi-species test that seeks to address this problem by using a battery of ten bacteria and a fungus. But there has been little independent evaluation of this test, and there is no published information on its sensitivity to pesticides. Here, we assess the performance of MARA using a range of toxicants including reference chemicals, fungicides and environmental samples. Mean MARA microbial toxic concentrations and IC20s (20% Inhibitory concentrations) indicate the toxicant concentrations affecting the more sensitive micro-organisms, while the mean IC50 (50% Inhibitory concentration) was found to be the concentration that was toxic to most MARA species. For the two fungicides tested, the yeast (Pichia anomalia) was the most sensitive of the ten MARA species, and was more sensitive than the nine other yeasts tested. The test may be particularly valuable for work with fungicides. Mean MARA IC50s were comparable to values for nine other yeast species and the lowest individual IC50s for each toxicant were comparable to reported IC50s for Daphnia magna, Selenastrum capricornutum and Microtox® bioassays. MARA organisms exhibited more variable sensitivities, with the most sensitive organism being different for different samples, enhancing the likelihood of toxicity detection and giving a toxicity “fingerprint” that may help identify toxicants. The test, therefore, has great potential and would be valuable for ecotoxicological testing of pollutants.
Starting Page	1626
Ending Page	1633
Page Count	8
File Format	PDF
ISSN	09639292
Journal	Ecotoxicology
Volume Number	19
Issue Number	8
e-ISSN	15733017
Language	English
Publisher	Springer US
Publisher Date	2010-09-30
Publisher Place	Boston
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Bacteria bioassays Fungicides Yeast growth inhibition Microscale toxicity tests Tetrazolium red Environmental Management Ecology Environment Ecotoxicology
Content Type	Text
Resource Type	Article
Subject	Health, Toxicology and Mutagenesis Medicine Toxicology Management, Monitoring, Policy and Law

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

An assessment of the potential of the microbial assay for risk assessment (MARA) for ecotoxicological testing.

Potential of the microbial assay for risk assessment (MARA) for assessing ecotoxicological effects of herbicides to non-target organisms

Potential of the microbial assay for risk assessment (MARA) for assessing ecotoxicological effects of herbicides to non-target organisms.

A test battery approach to the ecotoxicological evaluation of cadmium and copper employing a battery of marine bioassays

Biomarker assessment of toxicity with miniaturised bioassays: diclofenac as a case study

Evaluation of usefulness of Microbial Assay for Risk Assessment (MARA) in the cyanobacterial toxicity estimation

Ecotoxicological assessment of effluents in the Basque country (Northern Spain) by acute and chronic toxicity tests using Daphnia magna straus

Ecotoxicological characterization of a tropical soil after diazinon spraying

Assessment of heavy metals mobility and toxicity in contaminated sediments by sequential extraction and a battery of bioassays

An assessment of the potential of the microbial assay for risk assessment (MARA) for ecotoxicological testing

Similar Documents

An assessment of the potential of the microbial assay for risk assessment (MARA) for ecotoxicological testing.

Potential of the microbial assay for risk assessment (MARA) for assessing ecotoxicological effects of herbicides to non-target organisms

Potential of the microbial assay for risk assessment (MARA) for assessing ecotoxicological effects of herbicides to non-target organisms.

A test battery approach to the ecotoxicological evaluation of cadmium and copper employing a battery of marine bioassays

Biomarker assessment of toxicity with miniaturised bioassays: diclofenac as a case study

Evaluation of usefulness of Microbial Assay for Risk Assessment (MARA) in the cyanobacterial toxicity estimation

Ecotoxicological assessment of effluents in the Basque country (Northern Spain) by acute and chronic toxicity tests using Daphnia magna straus

Ecotoxicological characterization of a tropical soil after diazinon spraying

Assessment of heavy metals mobility and toxicity in contaminated sediments by sequential extraction and a battery of bioassays

An assessment of the potential of the microbial assay for risk assessment (MARA) for ecotoxicological testing