NDLI: Subcellular/tissue distribution and responses to oil exposure of the cytochrome P450-dependent monooxygenase system and glutathione S-transferase in freshwater prawns (Macrobrachium malcolmsonii, M. lamarrei lamarrei)

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Subcellular/tissue distribution and responses to oil exposure of the cytochrome P450-dependent monooxygenase system and glutathione S-transferase in freshwater prawns (Macrobrachium malcolmsonii, M. lamarrei lamarrei)

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Subcellular/tissue distribution and responses to oil exposure of the cytochrome P450-dependent monooxygenase system and glutathione S-transferase in freshwater prawns (Macrobrachium malcolmsonii, M. lamarrei lamarrei)

Content Provider	Springer Nature Link
Author	Arun, S. Rajendran, A. Subramanian, P.
Copyright Year	2006
Abstract	Subcellular fractions (mitochondrial, cytosolic and microsomal) prepared from the tissues (hepatopancreas, muscle and gill) of freshwater prawns Macrobrachium malcolmsonii and Macrobrachium lamarrei lamarrei were scrutinized to investigate the presence of mixed function oxygenase (MFO) and conjugating enzymes (glutathione-S-transferase, GST). Cytochrome P450 (CYP) and other components (cytochrome b5; NADPH-cytochrome c (CYP) reductase and NADH-cytochrome c-reductase activities) of the MFO system were predominantly present in the hepatic microsomal fraction of M. malcolmsonii and M. lamarrei lamarrei. The results are in agreement with the notion that monooxygenase system is mainly membrane bound in the endoplasmic reticulum, and that the hepatopancreas is the major metabolic tissue for production of biotransformation enzymes in crustaceans. Further, the prawns were exposed to two sublethal (0.9 ppt (parts per thousand) and 2.3 ppt) concentrations of oil effluent. At the end of 30th day, hydrocarbons and detoxifying enzymes were analysed in the hepatopancreas. The accumulations of hydrocarbon in the tissues gradually increased when exposed to sublethal concentrations of oil effluent and were associated with significantly enhanced levels of cytochrome P450 (180.6±6.34 pmol mg−1 protein (P<0.05 versus control, 136.5±7.1 pmol mg−1 protein) for 2.3 ppt and 305.6±8.5 pmol mg−1 protein (P<0.001 versus control, 132.3±6.8 pmol mg−1 protein] for 0.9 ppt of oil exposed M. malcolmsonii; 150±6.5 pmol mg−1 protein (P<0.01 versus control, 84.6±5.2 pmol mg−1 protein) for 2.3 ppt and 175±5.5 pmol mg−1 protein (P<0.01 versus control, 87.6±5.4 pmol mg−1 protein) for 0.9 ppt of oil exposed M. lamarrei lamarrei), NADPH cytochrome c-reductase activity (14.7±0.6 nmol min−1 mg−1 protein (P<0.05 versus control, 6.8±0.55 nmol min−1 mg−1 protein) for 2.3 ppt and 12.1±0.45 nmol min−1 mg−1 protein (P<0.01 versus control, 6.9±0.42 nmol min−1 mg−1 protein) for 0.9 ppt of oil exposed M. malcolmsonii; 12.5±0.31 nmol min−1 mg−1 protein (P<0.001 versus control, 4.6±0.45 nmol min−1 mg−1 protein) for 2.3 ppt and 9.6±0.32 nmol min−1 mg−1 protein (P<0.01 versus control, 4.9±0.41 nmol min−1 mg−1 protein) for 0.9 ppt of oil exposed M. lamarrei lamarrei) and cytochrome b5 (124.8±3.73 pmol mg−1 protein (P<0.01 versus control, 76.8±4.2 pmol mg−1 protein) for 2.3 ppt and 115.3±3.86 pmol mg−1 protein (P<0.01 versus control, 76.4±4.25 pmol mg−1 protein) for 0.9 ppt of oil exposed M. malcolmsonii and 110±3.11 pmol mg−1 protein (P<0.01 versus control, 63.7±3.24 pmol mg−1 protein) for 2.3 ppt and 95.3±2.63 pmol mg−1 protein (P<0.01 versus control, 61.4±2.82 pmol mg−1 protein) for 0.9 ppt of oil exposed M. lamarrei lamarrei). The enhanced levels of biotransformation enzymes in oil-exposed prawns demonstrate a well-established detoxifying mechanism in crustaceans, and the response offers the possibility of use as a biomarker for the early detection of oil pollution.
Starting Page	341
Ending Page	346
Page Count	6
File Format	PDF
ISSN	09639292
Journal	Ecotoxicology
Volume Number	15
Issue Number	4
e-ISSN	15733017
Language	English
Publisher	Kluwer Academic Publishers-Plenum Publishers
Publisher Date	2006-05-04
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Cytochrome P450 NAD(P)H-cytochrome c-reductase activities Cytochrome b5 Sucellular fraction M. malcolmsonii M. lamarre lamarrei Monitoring/Environmental Analysis/Environmental Ecotoxicology Environmental Management Environment Ecology
Content Type	Text
Resource Type	Article
Subject	Health, Toxicology and Mutagenesis Medicine Toxicology Management, Monitoring, Policy and Law

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Subcellular/tissue distribution and responses to oil exposure of the cytochrome P450-dependent monooxygenase system and glutathione S-transferase in freshwater prawns (Macrobrachium malcolmsonii, M. lamarrei lamarrei)

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