NDLI: Genetic Selection and Molecular Analysis of Domesticated Rainbow Trout for Enhanced Growth on Alternative Diet Sources

Content Provider	Springer Nature Link
Author	Overturf, Ken Bullock, Dan LaPatra, Scott Hardy, Ron
Copyright Year	2004
Abstract	Real-time polymerase chain reaction was used to monitor the expression of specific genes and confirm that an alteration in level of expression correlates with changes in diet, metabolism, or immune response. To test these probe and primer sets for their ability to monitor changes in gene expression, groups of fish were experimentally challenged either via daylength, vitamin availability, or with pathogenic microorganisms. The probes and primers sets were then used to analyze expression levels of specific gene products from isolated RNA. The expression of certain genes such as myosin in muscle correlated significantly with protein intake and seasonal daylength. Other genes involved with growth and metabolism such as insulin-like growth factor and pyruvate kinase showed lower levels of significance in correlating with planes of nutrition and varied with diet and treatment. However differences for pyruvate kinase were found in early tests done with animals receiving a vitamin-reduced diet. For factors relating immunological status after infection with microbiological pathogens, several factors such as MX-1 and CD-8 correlated with dose of the viral pathogen infectious hematopoeitic necrosis virus. Association of pathogen dose with immunological expression level was much less pronounced when fish were tested with bacterial microorganisms. This work will aid in evaluating the effects of diet on fish health and nutrient utilization and for evaluating the effects of selection of trout strains.
Starting Page	409
Ending Page	418
Page Count	10
File Format	PDF
ISSN	03781909
Journal	Environmental Biology of Fishes
Volume Number	69
Issue Number	1-4
e-ISSN	15735133
Language	English
Publisher	Kluwer Academic Publishers
Publisher Date	2004-01-01
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Hydrobiology Zoology Animal Systematics/Taxonomy/Biogeography Environment Nature Conservation
Content Type	Text
Resource Type	Article
Subject	Aquatic Science Ecology, Evolution, Behavior and Systematics

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Observations of Size-related Asymmetries in Diet and Energy Intake of Rainbow Trout in a Regulated River

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