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| Content Provider | Springer Nature Link |
|---|---|
| Author | Khallef, Messaouda Liman, Recep Konuk, Muhsin Ciğerci, İbrahim Hakkı Beuareth, Djameleddine Tabet, Mouna Abda, Ahlem |
| Copyright Year | 2013 |
| Abstract | Genotoxic effects of bromoform and chloroform, disinfection by-products of the chlorination of drinking water, were examined by using mitotic index (MI), mitotic phase, chromosome aberrations (CAs) and comet assay on root meristematic cells of Allium cepa. Different concentrations of bromoform (25, 50, 75 and 100 μg/mL) and chloroform (25, 50, 100 and 200 μg/mL) were introduced to onion tuber roots. Distilled water was used as a negative control and methyl methansulfonate (MMS-10 μg/mL) as positive control. All obtained data were subjected to statistical analyses by using SPSS 15.0 for Windows software. For comparison purposes, Duncan multiple range tests by using one-way analysis of variance were employed and p < 0.05 was accepted as significant value. Exposure of both chemicals (except 25 μg/mL applications of bromoform) significantly decreased MI. Bromoform and chloroform (except 25 μg/mL applications) increased total CAs in Allium anaphase-telophase test. A significant increase in DNA damage was also observed at all concentrations of both bromoform and chloroform examined by comet assay. The damages were higher than that of positive control especially at 75–100 μg/mL for bromoform and 100–200 μg/mL for chloroform. |
| Starting Page | 207 |
| Ending Page | 213 |
| Page Count | 7 |
| File Format | |
| ISSN | 09209069 |
| Journal | Cytotechnology |
| Volume Number | 67 |
| Issue Number | 2 |
| e-ISSN | 15730778 |
| Language | English |
| Publisher | Springer Netherlands |
| Publisher Date | 2013-12-21 |
| Publisher Place | Dordrecht |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Allium test Bromoform Chloroform Comet assay DNA damage Mitotic index Biotechnology Biomedicine general Biochemistry |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Clinical Biochemistry Bioengineering Biomedical Engineering Biotechnology |
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