NDLI: Serum-free transfection of CHO-cells with tailor-made unilamellar vesicles

Content Provider	Springer Nature Link
Author	Reisinger, Hannes Sevcsik, Eva Vorauer Uhl, Karola Lohner, Karl Katinger, Hermann Kunert, Renate
Copyright Year	2007
Abstract	At present, a number of transfection techniques are available to introduce foreign DNA into cells, but still minimal intrusion or interference with normal cell physiology, low toxicity, reproducibility, cost efficiency and successful creation of stable transfectants are highly desirable properties for improved transfection techniques.For all previous transfection experiments done in our labs, using serum-free cultivated host cell lines, an efficiency value of ∼0.1% for selection of stable cell lines has not been exceeded, consequently we developed and improved a transfection system based on defined liposomes, so-called large unilamellar vesicles, consisting of different lipid compositions to facilitate clone selection and increase the probability for creation of recombinant high-production clones. DNA and DOTAP/DOPE or CHEMS/DOPE interact by electrostatic means forming so-called lipoplexes (Even-Chen and Barenholz 2000) and the lipofection efficiency of those lipoplexes has been determined via confocal microscopy.In addition, the expression of the EGFP was determined by FACS to investigate transient as well as stable transfection and the transfection efficiency of a selection of different commercially available transfection reagents and kits has been compared to our tailor-made liposomes.
Starting Page	157
Ending Page	168
Page Count	12
File Format	PDF
ISSN	09209069
Journal	Cytotechnology
Volume Number	54
Issue Number	3
e-ISSN	15730778
Language	English
Publisher	Springer Netherlands
Publisher Date	2007-07-13
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	CHO-cells EGFP Liposomes SAXS analysis Serum-free transfection Transient transfection Biochemistry Biomedicine general Biotechnology
Content Type	Text
Resource Type	Article
Subject	Cell Biology Clinical Biochemistry Bioengineering Biomedical Engineering Biotechnology

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Serum-free transfection of CHO-cells with tailor-made unilamellar vesicles.

Serum-free transfection of CHO-cells with tailor-made unilamellar vesicles

Serum-free transfection of CHO cells with chemically defined transfection systems and investigation of their potential for transient and stable transfection

Serum-free transfection of CHO cells with chemically defined transfection systems and investigation of their potential for transient and stable transfection

Iron (III) citrate inhibits polyethylenimine-mediated transient transfection of Chinese hamster ovary cells in serum-free medium

Serum-free transfection of CHO cells with chemically defined transfection systems and investigation of their potential for transient and stable transfection.

Transient transfection induces different intracellular calcium signaling in CHO K1 versus HEK 293 cells

PEI/DNA formation affects transient gene expression in suspension Chinese hamster ovary cells via a one-step transfection process

Design of serum-free medium for suspension culture of CHO cells on the basis of general commercial media

Serum-free transfection of CHO-cells with tailor-made unilamellar vesicles

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Serum-free transfection of CHO-cells with tailor-made unilamellar vesicles.

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