NDLI: Effects of temperature shift on cell cycle, apoptosis and nucleotide pools in CHO cell batch cultues

Content Provider	Springer Nature Link
Author	Moore, Alison Mercer, Jennifer Dutina, George Donahue, Christopher J. Bauer, Kenneth D. Mather, Jennie P. Etcheverry, Tina Ryll, Thomas
Copyright Year	1997
Abstract	Temperature reduction in CHO cell batch culture may be beneficial in the production of recombinant protein and in maintenance of viability. The effects on cell cycle, apoptosis and nucleotide pools were studied in cultures initiated at 37°C and temperature shifted to 30 °C after 48 hours. In control cultures maintained at 37 °C, viable cells continued to proliferate until the termination of the culture, however, temperature reduction caused a rapid decrease in the percent of cells in S phase and accumulation of cells in G-1. This was accompanied by a concurrent reduction in U ratio (UTO/UDP-GNAc), previously shown to be a sensitive indicator of growth rate. Culture viability was extended following temperature shift, as a result of delayed onset of apoptosis, however, once initiated, the rate and manner of cell death was similar to that observed at 37 °C. All nucleotide pools were similarly degraded at the time of apoptotic cell death. Temperature reduction to 30 °C did not decrease the energy charge of the cells, however, the overall rate of metabolism was reduced. The latter may be sufficient to extend culture viability via a reduction in toxic metabolites and/or limitation of nutrient deprivation. However, the possibility remains that the benefits of temperature reduction in terms of both viability and productivity are more directly associated with cultures spending extended time in G-1.
Starting Page	47
Ending Page	54
Page Count	8
File Format	PDF
ISSN	09209069
Journal	Cytotechnology
Volume Number	23
Issue Number	1-3
e-ISSN	15730778
Language	English
Publisher	Kluwer Academic Publishers
Publisher Date	1997-01-01
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Biomedicine general Biochemistry Proteomics Cell Biology
Content Type	Text
Resource Type	Article
Subject	Cell Biology Clinical Biochemistry Bioengineering Biomedical Engineering Biotechnology

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Effects of temperature shift on cell cycle, apoptosis and nucleotide pools in CHO cell batch cultues

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