NDLI: Culture, characterization and differentiation of cells from buffalo (Bubalus bubalis) amnion

Content Provider	Springer Nature Link
Author	Mann, A. Yadav, R. P. Singh, J. Kumar, D. Singh, B. Yadav, P. S.
Copyright Year	2012
Abstract	Stem cells present an important tool in livestock assisted reproduction and veterinary therapeutic field such as tissue engineering. We report for the first time isolation of pluripotent stem cell-like cells expressing pluripotency markers (alkaline phospahatase, OCT-4, NANOG and SOX-2) from the amnion of water buffalo (Bubalus bubalis). The cells showed no apparent abnormalities in their chromosomal profiles before and after cryopreservation. The cytochemical staining revealed that pluripotent cells were capable of undergoing directed differentiation in vitro into osteocytes. It could be inferred that amnion-derived pluripotent stem cell-like cells can be isolated, cultured for many passages and differentiated into mesoderm lineage, and may be an alternative source to mesenchymal stem cells. These cells can have applications in assisted reproduction, developmental biological and regenerative medicine.
Starting Page	23
Ending Page	30
Page Count	8
File Format	PDF
ISSN	09209069
Journal	Cytotechnology
Volume Number	65
Issue Number	1
e-ISSN	15730778
Language	English
Publisher	Springer Netherlands
Publisher Date	2012-07-21
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Amnion cells Buffalo Differentiation Pluripotency Stem cells Biotechnology Biomedicine general Biochemistry
Content Type	Text
Resource Type	Article
Subject	Cell Biology Clinical Biochemistry Bioengineering Biomedical Engineering Biotechnology

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Culture, characterization and differentiation of cells from buffalo (Bubalus bubalis) amnion.

Culture, characterization and differentiation of cells from buffalo (Bubalus bubalis) amnion

Differential developmental competence and gene expression patterns in buffalo (Bubalus bubalis) nuclear transfer embryos reconstructed with fetal fibroblasts and amnion mesenchymal stem cells

Buffalo (Bubalus bubalis) term amniotic-membrane-derived cells exhibited mesenchymal stem cells characteristics in vitro

A comparative study on efficiency of adult fibroblasts and amniotic fluid-derived stem cells as donor cells for production of hand-made cloned buffalo (Bubalus bubalis) embryos

Differences in developmental competence and gene expression profiles between buffalo (Bubalus bubalis) preimplantation embryos cultured in three different embryo culture media

Differential developmental competence and gene expression patterns in buffalo (Bubalus bubalis) nuclear transfer embryos reconstructed with fetal fibroblasts and amnion mesenchymal stem cells.

The simulated microgravity enhances multipotential differentiation capacity of bone marrow mesenchymal stem cells

Selection of appropriate isolation method based on morphology of blastocyst for efficient derivation of buffalo embryonic stem cells

Culture, characterization and differentiation of cells from buffalo (Bubalus bubalis) amnion

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Culture, characterization and differentiation of cells from buffalo (Bubalus bubalis) amnion.

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