NDLI: Identification of the pivotal role of glutamate in enhancing insect cell growth using factor analysis

Content Provider	Springer Nature Link
Author	Chou, Cheng Chung
Copyright Year	2013
Abstract	Although the insect cell/baculovirus system is an important expression platform for recombinant protein production, our understanding of insect cell metabolism with respect to enhancing cell growth capability and productivity is still limited. Moreover, different host insect cell lines may have different growth characteristics associated with diverse product yields, which further hampers the elucidation of insect cell metabolism. To address this issue, the growth behaviors and utilization profiles of common metabolites among five cultured insect cell lines (derived from two insect hosts, Spodoptera frugiperda and Spodoptera exigua) were investigated in an attempt to establish a metabolic framework that can interpret the different cell growth behaviors. To analyze the complicated metabolic dataset, factor analysis was introduced to differentiate the crucial metabolic variations among these cells. Factor analysis was used to decompose the metabolic data to obtain the underlying factors with biological meaning that identify glutamate (a metabolic intermediate involved in glutaminolysis) as a key metabolite for insect cell growth. Notably, glutamate was consumed in significant amounts by fast-growing insect cell lines, but it was produced by slow-growing lines. A comparative experiment using cells grown in culture media with and without glutamine (the starting metabolite in glutaminolysis) was conducted to further confirm the pivotal role of glutamate. The factor analysis strategy allowed us to elucidate the underlying structure and inter-correlation between insect cell growth and metabolite utilization to provide some insights into insect cell growth and metabolism, and this strategy can be further extended to large-scale metabolomic analysis.
Starting Page	853
Ending Page	860
Page Count	8
File Format	PDF
ISSN	09209069
Journal	Cytotechnology
Volume Number	66
Issue Number	5
e-ISSN	15730778
Language	English
Publisher	Springer Netherlands
Publisher Date	2013-08-30
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Cell metabolism Factor analysis Glutamate Insect cell Biotechnology Biomedicine general Biochemistry
Content Type	Text
Resource Type	Article
Subject	Cell Biology Clinical Biochemistry Bioengineering Biomedical Engineering Biotechnology

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Identification of the pivotal role of glutamate in enhancing insect cell growth using factor analysis.

Identification of the pivotal role of glutamate in enhancing insect cell growth using factor analysis

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Identification of the pivotal role of glutamate in enhancing insect cell growth using factor analysis

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Identification of the pivotal role of glutamate in enhancing insect cell growth using factor analysis