NDLI: Selection of chemically defined media for CHO cell fed-batch culture processes

Content Provider	Springer Nature Link
Author	Pan, Xiao Streefland, Mathieu Dalm, Ciska Wijffels, René H. Martens, Dirk E.
Copyright Year	2016
Abstract	Two CHO cell clones derived from the same parental CHOBC® cell line and producing the same monoclonal antibody (BC-G, a low producing clone; BC-P, a high producing clone) were tested in four basal media in all possible combinations with three feeds (=12 conditions) in fed-batch cultures. Higher amino acid feeding did not always lead to higher mAb production. The two clones showed differences in cell physiology, metabolism and optimal medium-feed combinations. During the phase transitions of all cultures, cell metabolism showed a shift represented by lower specific consumption and production rates, except for the specific glucose consumption rate in cultures fed by Actifeed A/B. The BC-P clone fed by Actifeed A/B showed a threefold cell volume increase and an increase of the specific consumption rate of glucose in the stationary phase. Since feeding was based on glucose this resulted in accumulation of amino acids for this feed, while this did not occur for the poorer feed (EFA/B). The same feed also led to an increase of cell size for the BC-G clone, but to a lesser extent.
Starting Page	39
Ending Page	56
Page Count	18
File Format	PDF
ISSN	09209069
Journal	Cytotechnology
Volume Number	69
Issue Number	1
e-ISSN	15730778
Language	English
Publisher	Springer Netherlands
Publisher Date	2016-11-29
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Chinese hamster ovary (CHO) cell Cell culture medium Fed-batch Antibody production Metabolite profile Phase transition Biotechnology Biomedicine Biochemistry
Content Type	Text
Resource Type	Article
Subject	Cell Biology Clinical Biochemistry Bioengineering Biomedical Engineering Biotechnology

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