NDLI: Development of a Serum-free Suspension Process for the Production of a Conditionally Replicating Adenovirus using A549 Cells

Content Provider	Springer Nature Link
Author	Longley, Robert Radzniak, Laurie Santoro, Marc Tsao, Yung Shyeng Condon, Russell G. G. Lio, Peggy Voloch, Marcio Liu, Zhong
Copyright Year	2006
Abstract	Conditionally replicating adenoviruses (CRAVs) are a group of recombinant human adenoviruses genetically engineered to replicate in selected tissues, such as tumors. These viruses could potentially offer significant medicinal benefits, since the restrictive replication of these viral vectors leads to the lysis of target cells without harm to the surrounding tissues. The in vitro propagation and amplification of the CRAV vectors often requires special host cells with deregulated growth control pathways. In order to develop an efficient cell culture process for the scaleable production of a CRAV vector, A549 cells, a human lung carcinoma cell line normally cultured in adherent culture, were adapted to suspension culture. CRAV production was demonstrated with the suspension-adapted A549 cells and a baseline production process was developed in shake flasks. The ability to scale-up virus production was confirmed in stirred tank bioreactors. Molecular characterization of the suspension-adapted A549 cells indicates no significant changes in cellular mechanisms related to adenovirus infection.
Starting Page	161
Ending Page	171
Page Count	11
File Format	PDF
ISSN	09209069
Journal	Cytotechnology
Volume Number	49
Issue Number	2-3
e-ISSN	15730778
Language	English
Publisher	Kluwer Academic Publishers
Publisher Date	2006-06-12
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Adenovirus Recombinant adenovirus Gene therapy A549 cells Serum-free Suspension Conditionally replicating adenoviruses (CRAVs) Biotechnology Biomedicine general Biochemistry
Content Type	Text
Resource Type	Article
Subject	Cell Biology Clinical Biochemistry Bioengineering Biomedical Engineering Biotechnology

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Development of a Serum-free Suspension Process for the Production of a Conditionally Replicating Adenovirus using A549 Cells

Development of a Serum-free Suspension Process for the Production of a Conditionally Replicating Adenovirus using A549 Cells

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