NDLI: Adaptation of BHK cells producing a recombinant protein to serum-free media and protein-free medium

Content Provider	Springer Nature Link
Author	Cruz, Helder J. Moreira, José L. Stacey, Glyn Dias, Elsa M. Hayes, Katherine Looby, Dennis Griffiths, Bryan Carrondo, Manuel J. T.
Copyright Year	1998
Abstract	In this work a recombinant BHK21 clone producing a fusion protein with potential application in tumour target therapy was adapted to five different serum-free media (SFM) and to a protein-free medium (PFM). Only the PFM did not require a gradual adaptation to cell growth in the absence of serum. All tested SFM required a gradual adaptation (up to 35 days). For the majority of the SFM tested, cell specific productivity was not affected by the decrease in serum concentration during adaptation; however, cell growth was significantly affected by the serum decrease. Both cell growth and productivity were increased when PFM SMIF6 was used instead of the control medium. Long term measurements (approximately 100 days) of cell specific productivity for PFM and the two best SFM showed that productivity was maintained. This indicates the media capability to be used in long term production processes.
Starting Page	59
Ending Page	64
Page Count	6
File Format	PDF
ISSN	09209069
Journal	Cytotechnology
Volume Number	26
Issue Number	1
e-ISSN	15730778
Language	English
Publisher	Kluwer Academic Publishers
Publisher Date	1998-01-01
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Animal Anatomy / Morphology / Histology
Content Type	Text
Resource Type	Article
Subject	Cell Biology Clinical Biochemistry Bioengineering Biomedical Engineering Biotechnology

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