NDLI: An improved protocol for the isolation and cultivation of embryonic mouse myocytes

Content Provider	Springer Nature Link
Author	Rodgers, Laurel S. Schnurr, Daniel C. Broka, Derrick Camenisch, Todd D.
Copyright Year	2009
Abstract	In vitro cultures of cardiomyocytes have proven to be a useful tool for toxicological, pharmacological, and developmental studies, as well as for the study of the cellular and molecular mechanisms responsible for proper myocyte function. One deficient area of research is that of myocyte proliferation. Cardiomyocyte proliferation dramatically diminishes soon after birth and has a very limited occurrence within the adult heart, thus limiting the use of adult cells for proliferation studies. An improved understanding of the requirements for myocyte proliferation will allow for the development of better approaches to repair damaged heart tissue. Here, we provide a protocol for the reliable isolation of embryonic mouse myocytes. These myocytes behave similarly to those in vivo, including their ability to proliferate, providing an ideal system for the study of cardiomyocyte proliferation.
Starting Page	93
Ending Page	102
Page Count	10
File Format	PDF
ISSN	09209069
Journal	Cytotechnology
Volume Number	59
Issue Number	2
e-ISSN	15730778
Language	English
Publisher	Springer Netherlands
Publisher Date	2009-05-28
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Embryo Cardiomyocyte culture Myocardium Mouse Biochemistry Biotechnology Biomedicine general
Content Type	Text
Resource Type	Article
Subject	Cell Biology Clinical Biochemistry Bioengineering Biomedical Engineering Biotechnology

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