NDLI: Development of 13 EST-SSRs for Cerasus jamasakura and their transferability for Japanese flowering cherries

Content Provider	Springer Nature Link
Author	Tsuda, Yoshiaki Ue, Saneyoshi Kato, Shuri Katsuki, Toshio Mukai, Yuzuru Tsumura, Yoshihiko
Copyright Year	2008
Abstract	Simple sequence repeat (SSR) markers were developed for the flowering cherry Cerasus jamasakura (also known as Prunus jamasakura) using 31,995 expressed sequence tags (ESTs) from the NCBI database. Out of 96 of designed primer pairs, 63 showed clear PCR amplification and 13 of these revealed polymorphism in eight individuals sampled across the species’ range. The number of alleles detected and expected heterozygosity ranged from 1 to 8 and 0.000 to 0.833, respectively, when these 13 loci were examined in 23 individuals from a single population. For all except one of the lcoi, polymorphism was also detected in at least four of six other taxa of flowering cherries examined. The results show that the developed EST-SSRs are highly transferable, and that these markers are likely to be useful in studies of the population genetics of flowering cherries.
Starting Page	685
Ending Page	688
Page Count	4
File Format	PDF
ISSN	15660621
Journal	Conservation Genetics
Volume Number	10
Issue Number	3
e-ISSN	15729737
Language	English
Publisher	Springer Netherlands
Publisher Date	2008-05-29
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Cerasus jamasakura Expressed sequence tag Flowering cherry Prunoideae SSR Transferability Plant Sciences Evolutionary Biology Conservation Biology/Ecology Animal Anatomy / Morphology / Histology
Content Type	Text
Resource Type	Article
Subject	Genetics Ecology, Evolution, Behavior and Systematics

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Development of 14 EST-SSRs for Betula maximowicziana and their applicability to related species

Development of 11 EST-SSRs for Japanese white birch, Betula platyphylla var. japonica and their transferability to related species

Characterization of expressed sequence tag (EST)-derived microsatellite loci in the fire ant Solenopsis invicta (Hymenoptera: Formicidae)

Genetic structure of Cerasus jamasakura, a Japanese flowering cherry, revealed by nuclear SSRs: implications for conservation

Development of EST-SSRs in scallop (Patinopecten yessoensis) from sequence database

Development of EST-SSR markers from an inner bark cDNA library of Fagus crenata (Fagaceae)

Characterization of EST–SSRs from Cryptomeria japonica

Isolation and characterization of 39 novel polymorphic EST-SSR loci for the blood clam, Tegillarca granosa

Mining of EST-SSR from 454 pyrosequencing in the surf clam Mesodesma donacium (Lamark, 1818)

Development of 13 EST-SSRs for Cerasus jamasakura and their transferability for Japanese flowering cherries

Similar Documents

Development of 14 EST-SSRs for Betula maximowicziana and their applicability to related species

Development of 11 EST-SSRs for Japanese white birch, Betula platyphylla var. japonica and their transferability to related species

Characterization of expressed sequence tag (EST)-derived microsatellite loci in the fire ant Solenopsis invicta (Hymenoptera: Formicidae)

Genetic structure of Cerasus jamasakura, a Japanese flowering cherry, revealed by nuclear SSRs: implications for conservation

Development of EST-SSRs in scallop (Patinopecten yessoensis) from sequence database

Development of EST-SSR markers from an inner bark cDNA library of Fagus crenata (Fagaceae)

Characterization of EST–SSRs from Cryptomeria japonica

Isolation and characterization of 39 novel polymorphic EST-SSR loci for the blood clam, Tegillarca granosa

Mining of EST-SSR from 454 pyrosequencing in the surf clam Mesodesma donacium (Lamark, 1818)

Development of 13 EST-SSRs for Cerasus jamasakura and their transferability for Japanese flowering cherries