NDLI: Isolation and characterization of new microsatellite markers from the Japanese scallop (Patinopecten yessoensis)

Content Provider	Springer Nature Link
Author	Chen, Meng Chang, Ya Qing Ding, Jun Sun, Xiao Wen
Copyright Year	2009
Abstract	Twenty polymorphic microsatellite loci were isolated from the Japanese scallop (Patinopecten yessoensis) using a method of hybridizing twice. The genetic diversity was analyzed by these microsatellite markers in field specimens from Zhangzidao Island in Liaoning Province, China. Observed heterozygosity (H o), expected heterozygosity (H e), number of effective alleles (N e), and polymorphism information contents (PIC) were calculated. The heterozygote deficiency or excess was detected by the fixation index (Fis). The Hardy–Weinberg equilibrium was tested by possibilities (P HWE) using chi-square test. Results indicate that the average of allele numbers was 6.0. The mean values of the parameters H o, H e, N e, and PIC were 0.7921, 0.7076, 4.4347, and 0.6684, respectively. Only 4 of 20 microsatellite markers showed heterozygote deficiency including loci FJ262370, FJ262381, FJ262384, and FJ262400. The P HWE value indicates the wild population deviated somewhat from the Hardy–Weinberg equilibrium. These newly isolated markers increase the available molecular resources of the Japanese scallop.
Starting Page	1139
Ending Page	1142
Page Count	4
File Format	PDF
ISSN	15660621
Journal	Conservation Genetics
Volume Number	11
Issue Number	3
e-ISSN	15729737
Language	English
Publisher	Springer Netherlands
Publisher Date	2009-03-28
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Japanese scallop Microsatellite Population structure Twice hybridizations Plant Sciences Evolutionary Biology Conservation Biology/Ecology Animal Anatomy / Morphology / Histology
Content Type	Text
Resource Type	Article
Subject	Genetics Ecology, Evolution, Behavior and Systematics

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