NDLI: Cryptic species diversity in a widespread bumble bee complex revealed using mitochondrial DNA RFLPs

Content Provider	Springer Nature Link
Author	Murray, Tomás E. Fitzpatrick, Úna Brown, Mark J. F. Paxton, Robert J.
Copyright Year	2007
Abstract	Cryptic species diversity is thought to be common within the class Insecta, posing problems for basic ecological and population genetic studies and conservation management. Within the temperate bumble bee (Bombus spp.) fauna, members of the subgenus Bombus sensu stricto are amongst the most abundant and widespread. However, their species diversity is controversial due to the extreme difficulty or inability morphologically to identify the majority of individuals to species. Our character-based phylogenetic analyses of partial CO1 (700 bp) from 39 individuals spread across their sympatric European ranges provided unequivocal support for five taxa (3–22 diagnostic DNA base pair sites per species). Inclusion of 20 Irish specimens to the dataset revealed ≥2.3% sequence divergence between taxa and ≤1.3% within taxa. We developed a PCR-RFLP based method for unequivocally distinguishing amongst the four cryptic European taxa of this subgenus, B. cryptarum, B. lucorum, B. magnus and B. terrestris, and used it to analyse 391 females of the former three species collected across Ireland, all of which could be unambiguously assigned to species. Bombus lucorum was the most widely distributed and abundant of the cryptarum–lucorum–magnus species complex, comprising 56% of individuals, though it was significantly less abundant at higher altitudes (>200 m) whilst B. cryptarum was relatively more abundant at higher altitudes. Bombus magnus was rarely encountered at urban sites. Both B. lucorum and B. terrestris are nowadays reared commercially for pollination and transported globally. Our RFLP approach to identify native fauna can underpin ecological studies of these important cryptic species as well as the impact of commercial bumble bees on them.
Starting Page	653
Ending Page	666
Page Count	14
File Format	PDF
ISSN	15660621
Journal	Conservation Genetics
Volume Number	9
Issue Number	3
e-ISSN	15729737
Language	English
Publisher	Springer Netherlands
Publisher Date	2007-08-07
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Bombus Cytochrome c oxidase subunit 1 CO1 PCR-RFLP DNA barcode Evolutionary Biology Plant Sciences Animal Anatomy / Morphology / Histology Human Genetics
Content Type	Text
Resource Type	Article
Subject	Genetics Ecology, Evolution, Behavior and Systematics

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

PCR–RFLP technique for species identification of molted feathers in six species of co-occurring Ardeids

Microsatellite analysis supports the existence of three cryptic species within the bumble bee Bombus lucorum sensu lato

DNA-barcoding evidence for widespread introductions of a leech from the South AmericanHelobdella triserialis complex

A cryptic and critically endangered species revealed by mitochondrial DNA analyses: the Western Ground Parrot

Species-specific primers for the molecular identification of cryptic Bombus species in New Zealand

Diversity and distribution of cryptic species within theMugil cephalusspecies complex in Vietnam

DNA Barcode of Seven Indonesian Hornbills Species (Aves: Bucerotidae) Based on Mitochondrial DNA Cytochrome Oxidase Subunit I

Mitochondrial DNA analysis reveals genetic structure in two New Zealand Cook’s petrel (Pterodroma cookii) populations

Landscape heterogeneity predicts gene flow in a widespread polymorphic bumble bee, Bombus bifarius (Hymenoptera: Apidae)

Cryptic species diversity in a widespread bumble bee complex revealed using mitochondrial DNA RFLPs

Similar Documents

PCR–RFLP technique for species identification of molted feathers in six species of co-occurring Ardeids

Microsatellite analysis supports the existence of three cryptic species within the bumble bee Bombus lucorum sensu lato

DNA-barcoding evidence for widespread introductions of a leech from the South AmericanHelobdella triserialis complex

A cryptic and critically endangered species revealed by mitochondrial DNA analyses: the Western Ground Parrot

Species-specific primers for the molecular identification of cryptic Bombus species in New Zealand

Diversity and distribution of cryptic species within theMugil cephalusspecies complex in Vietnam

DNA Barcode of Seven Indonesian Hornbills Species (Aves: Bucerotidae) Based on Mitochondrial DNA Cytochrome Oxidase Subunit I

Mitochondrial DNA analysis reveals genetic structure in two New Zealand Cook’s petrel (Pterodroma cookii) populations

Landscape heterogeneity predicts gene flow in a widespread polymorphic bumble bee, Bombus bifarius (Hymenoptera: Apidae)

Cryptic species diversity in a widespread bumble bee complex revealed using mitochondrial DNA RFLPs