NDLI: Isolation and characterization of 24 microsatellite loci in Paphiopedilum rothschildianum, an endangered slipper orchid

Content Provider	Springer Nature Link
Author	Rodrigues, K. F. Kumar, S. V.
Copyright Year	2008
Abstract	This paper describes the characterization of 24 polymorphic microsatellite loci in Paphiopedilum rothschildianum, an endangered slipper orchid endemic to Sabah, Malaysia. A total of 36 clones containing microsatellite repeat motifs yielded 41 dinucleotide, 20 tetranucleotide and 30 cryptic simple repeat sequences. Thirty microsatellite loci were randomly selected to characterize the population, of which 24 were found to be polymorphic. The expected heterozygosity ranged from 0.0813 to 0.7279 (mean 0.4533) and the observed heterozygosity ranged from 0 to 1 (mean 0.3800). The results indicated that there was limited migration between the three subpopulations and a significant degree of genetic differentiation (FST = 0.5098) between populations implying that gene flow has been disrupted as a result of habitat fragmentation. The molecular markers have a potential application in population management, CITES enforcement and the forensic detection of specimens in international trade.
Starting Page	127
Ending Page	130
Page Count	4
File Format	PDF
ISSN	15660621
Journal	Conservation Genetics
Volume Number	10
Issue Number	1
e-ISSN	15729737
Language	English
Publisher	Springer Netherlands
Publisher Date	2008-02-14
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Paphiopedilum rothschildianum Microsatellite 5′ anchored PCR Heterozygosity CITES Animal Anatomy / Morphology / Histology Evolutionary Biology Human Genetics Plant Sciences
Content Type	Text
Resource Type	Article
Subject	Genetics Ecology, Evolution, Behavior and Systematics

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