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| Content Provider | Springer Nature Link |
|---|---|
| Author | Nielsen, Boye Schnack Jørgensen, Stine Fog, Jacob Ulrik Søkilde, Rolf Christensen, Ib Jarle Hansen, Ulla Brünner, Nils Baker, Adam Møller, Søren Nielsen, Hans Jørgen |
| Copyright Year | 2010 |
| Abstract | Approximately 25% of all patients with stage II colorectal cancer will experience recurrent disease and subsequently die within 5 years. MicroRNA-21 (miR-21) is upregulated in several cancer types and has been associated with survival in colon cancer. In the present study we developed a robust in situ hybridization assay using high-affinity Locked Nucleic Acid (LNA) probes that specifically detect miR-21 in formalin-fixed paraffin embedded (FFPE) tissue samples. The expression of miR-21 was analyzed by in situ hybridization on 130 stage II colon and 67 stage II rectal cancer specimens. The miR-21 signal was revealed as a blue chromogenic reaction, predominantly observed in fibroblast-like cells located in the stromal compartment of the tumors. The expression levels were measured using image analysis. The miR-21 signal was determined as the total blue area (TB), or the area fraction relative to the nuclear density (TBR) obtained using a red nuclear stain. High TBR (and TB) estimates of miR-21 expression correlated significantly with shorter disease-free survival (p = 0.004, HR = 1.28, 95% CI: 1.06–1.55) in the stage II colon cancer patient group, whereas no significant correlation with disease-free survival was observed in the stage II rectal cancer group. In multivariate analysis both TB and TBR estimates were independent of other clinical parameters (age, gender, total leukocyte count, K-RAS mutational status and MSI). We conclude that miR-21 is primarily a stromal microRNA, which when measured by image analysis identifies a subgroup of stage II colon cancer patients with short disease-free survival. |
| Starting Page | 27 |
| Ending Page | 38 |
| Page Count | 12 |
| File Format | |
| ISSN | 02620898 |
| Journal | Clinical & Experimental Metastasis |
| Volume Number | 28 |
| Issue Number | 1 |
| e-ISSN | 15737276 |
| Language | English |
| Publisher | Springer Netherlands |
| Publisher Date | 2010-10-31 |
| Publisher Place | Dordrecht |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | MicroRNA MiR-21 Colorectal cancer In situ hybridization LNA Surgical Oncology Hematology Oncology Biomedicine general Cancer Research |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cancer Research Oncology |
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