NDLI: Galectin-3 germline variant at position 191 enhances nuclear accumulation and activation of β-catenin in gastric cancer

Content Provider	Springer Nature Link
Author	Kim, Seok Jun Shin, Ji Young Cheong, Teak Chin Choi, Il Ju Lee, Yeon Su Park, Seok Hee Chun, Kyung Hee
Copyright Year	2011
Abstract	Mutation of galectin-3 at position 191 (rs4644) substituting proline to histidine (gal-3H64) resulted in the acquisition of resistance to drug-induced apoptosis by breast cancer cells. This study employed gastric cancer cells and patient tissues in attempts to elucidate how and why this mutation in galectin-3 (gal-3H64) enhances cancer progression, compared to wild type galectin-3 (gal-3P64). First, we prepared lenti-virus constructs containing gal-3P64, gal-3H64 and LacZ, and used them to infect galectin-3 null SNU-638 cells. We found that gal-3H64 over-expression increases gastric cancer cell growth more than gal-3P64 or LacZ over-expression. Also, gal-3H64 over-expression conferred more resistance to cisplatin or 5-FU induced cytotoxicity than gal-3P64. Gal-3H64 also enhanced nuclear accumulation of β-catenin as well as increased expression of TCF-4 target genes, such as fascin-1 and c-Myc through the augmented promoter binding activity of TCF-4, than gal-3P64. We also demonstrated stronger staining of β-catenin and galectin-3 in malignant tissues from gastric cancer patients with mutated galectin-3 at position 191 (gal-3 191) (A/A) (H64) and greater localization in the nucleus than in gal-3 191 A/C (P64) cancer patients. Taken together, we elucidated in this study that germline variant of gal-3H64 increases nuclear accumulation of β-catenin and promotes TCF transcriptional activity and enhances more the galectin-3’s role in gastric cancer progression.
Starting Page	743
Ending Page	750
Page Count	8
File Format	PDF
ISSN	02620898
Journal	Clinical & Experimental Metastasis
Volume Number	28
Issue Number	8
e-ISSN	15737276
Language	English
Publisher	Springer Netherlands
Publisher Date	2011-07-13
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Galectin-3P64 Galectin-3H64 β-Catenin Tumor progression Gastric cancer Surgical Oncology Biomedicine general Oncology Cancer Research Hematology
Content Type	Text
Resource Type	Article
Subject	Cancer Research Oncology

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