NDLI: Arylsulfatase B regulates colonic epithelial cell migration by effects on MMP9 expression and RhoA activation

Content Provider	Springer Nature Link
Author	Bhattacharyya, Sumit Tobacman, Joanne K.
Copyright Year	2009
Abstract	Arylsulfatase B (ASB; N-acetylgalactosamine-4-sulfatase; 4-sulfatase; ARSB) is the enzyme that removes 4-sulfate groups from N-acetylgalactosamine 4-sulfate, which combines with glucuronate to form the disaccharide unit of chondroitin-4-sulfate (C4S). In this study, we report how variation in expression of ASB affected the migration of human colonic epithelial cells. In the T84 cell line, derived from lung metastasis of malignant colonic epithelial cells, the activity of ASB, as well as steroid sulfatase, arylsulfatase A, and galactose-6-sulfatase, were significantly less than in normal, primary colonic epithelial cells and in the NCM460 cell line which was derived from normal colonocytes. In the T84 cells, matrix metalloproteinase 9 (MMP9), activated RhoA, and cell migration, as well as C4S content, were significantly more than in the NCM460 cells. Silencing and overexpression of ASB had inverse effects on MMP9, activated RhoA, and cell migration, as well as the C4S content, in the NCM460 and T84 cells. When ASB expression was silenced by siRNA in the NCM460 cells, MMP9 secretion increased to over 3 times the basal level, activated RhoA increased ~85%, and cell migration increased ~52%. Following overexpression of ASB, MMP9 declined 51%, activated RhoA declined ~51%, and cell migration decreased ~37%. These findings demonstrate marked effects of ASB expression on the migratory activity of colonic epithelial cells, activated RhoA, and MMP9, and suggest a potential vital role of ASB, due to its impact on chondroitin sulfation, on determination of the invasive phenotype of colonic epithelial cells.
Starting Page	535
Ending Page	545
Page Count	11
File Format	PDF
ISSN	02620898
Journal	Clinical & Experimental Metastasis
Volume Number	26
Issue Number	6
e-ISSN	15737276
Language	English
Publisher	Springer Netherlands
Publisher Date	2009-03-22
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	ASB Chondroitin-4-sulfate Colonic epithelial cells Extracellular matrix MMP9 Migration RhoA Surgical Oncology Hematology Oncology Biomedicine general Cancer Research
Content Type	Text
Resource Type	Article
Subject	Cancer Research Oncology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Arylsulfatase B Regulates Interaction of Chondroitin 4-Sulfate and Kininogen in Renal Epithelial Cells

Leading malignant cells initiate collective epithelial cell invasion in a three-dimensional heterotypic tumor spheroid model

Increased Expression of Colonic Wnt9A through Sp1-mediated Transcriptional Effects involving Arylsulfatase B, Chondroitin 4-Sulfate, and Galectin-3

Functional blocking of specific integrins inhibit colonic cancer migration

Src kinase promotes adhesion-independent activation of FAK and enhances cellular migration in tamoxifen-resistant breast cancer cells

Transcriptional profiling identifies genes induced by hepatocyte-derived extracellular matrix in metastatic human colorectal cancer cell lines

Radiation Effects on Brain Extracellular Matrix

Matrix metalloproteinases stimulate epithelial-mesenchymal transition during tumor development

Mapping proteolytic cancer cell-extracellular matrix interfaces

Arylsulfatase B regulates colonic epithelial cell migration by effects on MMP9 expression and RhoA activation

Similar Documents

Arylsulfatase B Regulates Interaction of Chondroitin 4-Sulfate and Kininogen in Renal Epithelial Cells

Leading malignant cells initiate collective epithelial cell invasion in a three-dimensional heterotypic tumor spheroid model

Increased Expression of Colonic Wnt9A through Sp1-mediated Transcriptional Effects involving Arylsulfatase B, Chondroitin 4-Sulfate, and Galectin-3

Functional blocking of specific integrins inhibit colonic cancer migration

Src kinase promotes adhesion-independent activation of FAK and enhances cellular migration in tamoxifen-resistant breast cancer cells

Transcriptional profiling identifies genes induced by hepatocyte-derived extracellular matrix in metastatic human colorectal cancer cell lines

Radiation Effects on Brain Extracellular Matrix

Matrix metalloproteinases stimulate epithelial-mesenchymal transition during tumor development

Mapping proteolytic cancer cell-extracellular matrix interfaces

Arylsulfatase B regulates colonic epithelial cell migration by effects on MMP9 expression and RhoA activation