NDLI: Living in shear: platelets protect cancer cells from shear induced damage

Content Provider	Springer Nature Link
Author	Egan, Karl Cooke, Niamh Kenny, Dermot
Copyright Year	2014
Abstract	Pharmacologically and genetically induced thrombocytopenia is associated with decreased metastasis, highlighting the importance of platelets in the bloodborne dissemination of cancer cells. It is frequently suggested that platelets support metastasis, in part, by protecting cancer cells from shear stress, a biomechanical force generated by blood flow. However, there is currently no evidence to support this hypothesis. To address this, we investigated the effect of shear stress on A2780 ovarian cancer cells in the presence and absence of platelets. Using a cone and plate viscometer, suspensions of A2780 cells with and without platelets were exposed to shear rates representing venous (200 s−1) and arterial (1,500 s−1) blood flow. Lactate dehydrogenase (LDH) release was used to quantify shear induced membrane damage. Both venous and arterial shear rates induced the release of LDH from A2780 cells, demonstrating their susceptibility to shear forces. In contrast, platelets released minimal levels of LDH in response to similar conditions. In the presence of platelets, there was a significant decrease in LDH release by A2780 cells under shear conditions, suggesting that platelets can confer protection against shear induced damage. The disruption of platelet–cancer cell interactions could increase the shear stress induced destruction of cancer cells in vivo.
Starting Page	697
Ending Page	704
Page Count	8
File Format	PDF
ISSN	02620898
Journal	Clinical & Experimental Metastasis
Volume Number	31
Issue Number	6
e-ISSN	15737276
Language	English
Publisher	Springer Netherlands
Publisher Date	2014-06-19
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Metastasis Platelets Shear stress Membrane damage Cancer Research Biomedicine general Oncology Hematology Surgical Oncology
Content Type	Text
Resource Type	Article
Subject	Cancer Research Oncology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Metformin may protect nondiabetic breast cancer women from metastasis

Activated platelets enhance ovarian cancer cell invasion in a cellular model of metastasis

Beware of NK cells in pre-clinical metastasis models

Melanoma cell metastasis via P-selectin-mediated activation of acid sphingomyelinase in platelets

Shear Stress Induced Release of Von Willebrand Factor and Thrombospondin-1 in Uvec Extracellular Matrix Enhances Breast Tumour Cell Adhesion

Is gastrointestinal dysfunction induced by gastric cancer peritoneal metastasis relevant to impairment of interstitial cells of Cajal?

Dendritic cells recruitment in melanoma metastasis treated by electrochemotherapy

Differential changes in platelet VEGF, Tsp, CXCL12, and CXCL4 in patients with metastatic cancer

Endoglin suppresses human prostate cancer metastasis

Living in shear: platelets protect cancer cells from shear induced damage

Similar Documents

Metformin may protect nondiabetic breast cancer women from metastasis

Activated platelets enhance ovarian cancer cell invasion in a cellular model of metastasis

Beware of NK cells in pre-clinical metastasis models

Melanoma cell metastasis via P-selectin-mediated activation of acid sphingomyelinase in platelets

Shear Stress Induced Release of Von Willebrand Factor and Thrombospondin-1 in Uvec Extracellular Matrix Enhances Breast Tumour Cell Adhesion

Is gastrointestinal dysfunction induced by gastric cancer peritoneal metastasis relevant to impairment of interstitial cells of Cajal?

Dendritic cells recruitment in melanoma metastasis treated by electrochemotherapy

Differential changes in platelet VEGF, Tsp, CXCL12, and CXCL4 in patients with metastatic cancer

Endoglin suppresses human prostate cancer metastasis

Living in shear: platelets protect cancer cells from shear induced damage