NDLI: The identification of chromosome abnormalities associated with the invasive phenotype of uveal melanoma in vitro

Please wait, while we are loading the content...

Establishment and characterization of three novel human gastric cancer cell lines with differentiated intestinal phenotype derived from liver metastasis

Benzo-[a]-pyrene increases invasion in MDA-MB-231 breast cancer cells via increased COX-II expression and prostaglandin E2 (PGE2) output

Trefoil factor family-3 is associated with aggressive behavior of colon cancer cells

Distinct and opposite roles for SH2 and SH3 domains of v-src in embryo survival and hemangiosarcoma formation

Statistical kinematics of axillary nodal metastases in breast carcinoma

An Orthotopic Model of Lung Cancer to Analyze Primary and Metastatic NSCLC Growth in Integrin α1-Null Mice

Clinical & Experimental Metastasis : Volume 22, Issue 1, March 2005

Clinical & Experimental Metastasis : Volume 21

Clinical & Experimental Metastasis : Volume 20

Clinical & Experimental Metastasis : Volume 19

Clinical & Experimental Metastasis : Volume 18

Clinical & Experimental Metastasis : Volume 17

Clinical & Experimental Metastasis : Volume 16

Clinical & Experimental Metastasis : Volume 15

The identification of chromosome abnormalities associated with the invasive phenotype of uveal melanoma in vitro

Content Provider	Springer Nature Link
Author	Cross, Neil A. Rennie, Ian G. Murray, Anna K. Sisley, Karen
Copyright Year	2005
Abstract	Tumour cell cultures are often highly heterogeneous, containing sub-populations of cells with differing characteristics. To identify chromosome abnormalities that are associated with the invasive phenotype, we isolated highly invasive uveal melanoma cell populations using the Transwell assay. Using this invasion assay, invasive sub-populations of primary uveal melanoma short-term cultures, and an established cell line, were specifically isolated. A series of sequential assays were undertaken to enrich the invasive population, and the enhanced invasive ability was confirmed by Transwell invasion assay. Chromosome abnormalities in invasive and parental cells were identified by karyotyping and confirmed by comparative genome hybridisation. Invasive sub-populations of uveal melanoma cells were isolated from 3 uveal melanoma short term cultures and a uveal melanoma cell line. In all cases, invasive sub-populations had either acquired additional chromosome abnormalities to those present in the parental cell line, or other abnormalities present in the parental lines were lost. In the established cell line (SOM 157), invasive cells were characterised by widespread chromosomal instability, frequent telomere associations and additional copies of chromosome 20. The invasive phenotype of SOM 196 associated with the presence of a derivative chromosome 5, der(5)t(5;11)(q35;q12) whilst a translocation t(17;20)(q12;q13) was predominant amongst non-invasive cells. In two additional cultures, deletions on chromosome 6q were associated with reduced invasive ability. In conclusion, highly invasive populations of uveal melanoma cells demonstrate chromosomal abnormalities that differ from non-invasive cells. These include chromosome instability and abnormalities of chromosome 20, observations echoing those seen in metastatic uveal melanoma.
Starting Page	107
Ending Page	113
Page Count	7
File Format	PDF
ISSN	02620898
Journal	Clinical & Experimental Metastasis
Volume Number	22
Issue Number	2
e-ISSN	15737276
Language	English
Publisher	Kluwer Academic Publishers
Publisher Date	2005-01-01
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	chromosome instability clonal evolution cytogenetic invasion assay uveal melanoma Cancer Research Oncology Hematology Surgical Oncology
Content Type	Text
Resource Type	Article
Subject	Cancer Research Oncology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Involvement of HIF-1 in Invasion of Mum2B Uveal Melanoma Cells

Molecular determinants of human uveal melanoma invasion and metastasis

Acid treatment of melanoma cells selects for invasive phenotypes

The vascular phenotype of melanoma metastasis

Transcriptional profiling of human uveal melanoma from cell lines to intraocular tumors to metastasis

Single-cell tumor dormancy model of uveal melanoma

Establishment of an in vitro assay to measure the invasion of ovarian carcinoma cells through mesothelial cell monolayers

Mechanisms of resistance to imatinib mesylate in KIT-positive metastatic uveal melanoma

Host pigment epithelium-derived factor (PEDF) prevents progression of liver metastasis in a mouse model of uveal melanoma

The identification of chromosome abnormalities associated with the invasive phenotype of uveal melanoma in vitro

Similar Documents

Involvement of HIF-1 in Invasion of Mum2B Uveal Melanoma Cells

Molecular determinants of human uveal melanoma invasion and metastasis

Acid treatment of melanoma cells selects for invasive phenotypes

The vascular phenotype of melanoma metastasis

Transcriptional profiling of human uveal melanoma from cell lines to intraocular tumors to metastasis

Single-cell tumor dormancy model of uveal melanoma

Establishment of an in vitro assay to measure the invasion of ovarian carcinoma cells through mesothelial cell monolayers

Mechanisms of resistance to imatinib mesylate in KIT-positive metastatic uveal melanoma

Host pigment epithelium-derived factor (PEDF) prevents progression of liver metastasis in a mouse model of uveal melanoma

The identification of chromosome abnormalities associated with the invasive phenotype of uveal melanoma in vitro