NDLI: Subnuclear targeting of the RNA-binding motif protein RBM6 to splicing speckles and nascent transcripts

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Subnuclear targeting of the RNA-binding motif protein RBM6 to splicing speckles and nascent transcripts

Content Provider	Springer Nature Link
Author	Heath, Emma Sablitzky, Fred Morgan, Garry T.
Copyright Year	2010
Abstract	RNA-binding motif (RBM) proteins comprise a large family of RNA-binding proteins whose functions are poorly understood. Since some RBM proteins are candidate alternative splicing factors we examined whether one such member of the family, RBM6, exhibited a pattern of nuclear distribution and targeting consistent with this role. Using antibodies raised against mouse RBM6 to immmunostain mammalian cell lines we found that the endogenous protein was both distributed diffusely in the nucleus and concentrated in a small number of nuclear foci that corresponded to splicing speckles/interchromatin granule clusters (IGCs). Tagged RBM6 was also targeted to IGCs, although it accumulated in large bodies confined to the IGC periphery. The basis of this distribution pattern was suggested by the targeting of tagged RBM6 in the giant nuclei (or germinal vesicles (GVs)) of Xenopus oocytes. In spread preparations of GV contents RBM6 was localized both to lampbrush chromosomes and to the surface of many oocyte IGCs, where it was confined to up to 50 discrete patches. Each patch of RBM6 labelling corresponded to a bead-like structure of 0.5–1 μm diameter that assembled de novo on the IGC surface. Assembly of these novel structures depended on the repetitive N-terminal region of RBM6, which acts as a multimerization domain. Without this domain, RBM6 was no longer excluded from the IGC interior but accumulated homogeneously within it. Assembly of IGC-surface structures in mammalian cell lines also depended on the oligomerization domain of RBM6. Oligomerization of RBM6 also had morphological effects on its other major target in GVs, namely the arrays of nascent transcripts visible in lampbrush chromosome transcription units. The presence of oligomerized RBM6 on many lampbrush loops caused them to appear as dense structures with a spiral morphology that appeared quite unlike normal, extended loops. This distribution pattern suggests a new role for RBM6 in the co-transcriptional packaging or processing of most nascent transcripts.
Starting Page	851
Ending Page	872
Page Count	22
File Format	PDF
ISSN	09673849
Journal	Chromosome Research
Volume Number	18
Issue Number	8
e-ISSN	15736849
Language	English
Publisher	Springer Netherlands
Publisher Date	2010-11-18
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Lampbrush chromosome nuclear body interchromatin granule cluster germinal vesicle splicing factor Plant Genetics & Genomics Animal Genetics and Genomics Human Genetics Cell Biology
Content Type	Text
Resource Type	Article
Subject	Genetics

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