NDLI: Single-cell c-myc gene expression in relationship to nuclear domains

Content Provider	Springer Nature Link
Author	Bártová, Eva Harničarová, Andrea Krejčí, Jana Strašák, Luděk Kozubek, Stanislav
Copyright Year	2008
Abstract	Nuclear locations of the c-myc gene and its transcripts (c-myc T) have been investigated in relation to nuclear domains involved in RNA synthesis and processing. Transcription of the c-myc gene appears to be linked to the late G1- and preferentially to S-phases of the cell cycle. The c-myc gene and its transcripts were positioned non-randomly within the interphase nucleus; additionally, c-myc RNA signals accumulated at nucleoli. Using oligo-probes, designed to exon II and exon III of the c-myc gene, single c-myc T was preferentially observed in human carcinoma HT29 and A549 cells. Conversely, human embryonal teratocarcinoma NTERA cells were characterized by the presence of multiple c-myc RNA signals located in both the nucleoli and nucleoplasm. When accumulated at nucleoli, c-myc T occupied the periphery of this organelle, though not those associated with the cultivation surface. In HT29 cells, approximately 80% of c-myc T co-localized with the RNAP II positive regions, so-called transcription factories. However, in ∼20% of the cells with c-myc transcripts, the c-myc T was released from the site of synthesis, and was not associated with either transcription factories or SC35 domains. In ∼60% of nuclei with c-myc T, these signals were located in close proximity to the SC35 regions, but promyelocytic leukaemia bodies were associated with c-myc T only in ∼20% of the nuclei. Taken together, c-myc RNA signals were positioned in the most internal parts of the cell nuclei preferentially associated with the nucleoli. Specific nuclear and nucleolar positioning probably reflects the kinetics of c-myc RNA metabolism.
Starting Page	325
Ending Page	343
Page Count	19
File Format	PDF
ISSN	09673849
Journal	Chromosome Research
Volume Number	16
Issue Number	2
e-ISSN	15736849
Language	English
Publisher	Springer Netherlands
Publisher Date	2008-03-07
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	c-myc gene nuclear speckles nucleolus promyelocytic leukaemia body transcription site Cell Biology Human Genetics Animal Genetics and Genomics Plant Genetics & Genomics
Content Type	Text
Resource Type	Article
Subject	Genetics

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