NDLI: Molecular characterization of a putative heat shock protein cognate gene in Rhynchosciara americana

Content Provider	Springer Nature Link
Author	Andrade, Alexandre Siviero, Fabio Rezende Teixeira, Paula Santelli, Roberto Vicente Machado Santelli, Glaucia Maria
Copyright Year	2009
Abstract	An hsc70 homologue gene (Rahsc70) of the diptera Rhynchosciara americana was isolated and characterized. We were able to determine the mRNA sequence from an EST of salivary gland cDNA library, and a Rahsc70 cDNA cassette was used as a probe to isolate the genomic region from a genomic library. The mRNA expression of this gene parallels the 2B puff expansion, suggesting its involvement in protein processing, since this larval period corresponds to a high synthetic activity period. During heat shock stress conditions, hsc70 expression decreased. In situ hybridization of polytene chromosomes showed that the Rahsc70 gene is located near the C3 DNA puff. The cellular localization of Hsc70 protein showed this protein in the cytoplasm and in the nucleus.
Starting Page	935
Ending Page	945
Page Count	11
File Format	PDF
ISSN	09673849
Journal	Chromosome Research
Volume Number	17
Issue Number	7
e-ISSN	15736849
Language	English
Publisher	Springer Netherlands
Publisher Date	2009-09-19
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Rhynchosciara americana polytene chromosome DNA puff heat shock protein development Plant Genetics & Genomics Animal Genetics and Genomics Human Genetics Cell Biology
Content Type	Text
Resource Type	Article
Subject	Genetics

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Molecular characterization of a putative heat shock protein cognate gene in Rhynchosciara americana