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| Content Provider | Springer Nature Link |
|---|---|
| Author | Yamada, Daisuke Koyama, Yoshiyuki Komatsubara, Mai Urabe, Mayuko Mori, Masako Hashimoto, Yoichi Nii, Ryosuke Kobayashi, Midori Nakamoto, Ayako Ogihara, Jun Kato, Jun Mizu, Shigeki |
| Copyright Year | 2004 |
| Abstract | In order to seek chicken W chromosome-linked genes expressed significantly earlier than the time of gonadal differentiation, female-minus-male-subtracted cDNA macroarrays were prepared from day 2 (Hamburger-Hamilton stages 12-13), day 3 (stages 19-20) and day 4 (stages 24-25) embryos. From a total of 15-744 macroarrayed cDNA clones, 610 clones exhibiting significantly female-specific expression were selected. When each one of the 610 cDNA clones was used as a probe in Southern blot hybridization with male or female chicken genomic DNA, 62 clones, grouped into eight (A-H) types according to their patterns of hybridization, were considered to be derived from W chromosome-linked genes. When representative cDNA clones in each type were sequenced, clones derived from two known W-linked genes; SPIN-W and ATP5A1W, and from two hitherto unknown W-linked genes, represented by 2d-2D9 and 2d-2F9 clones, were identified and their localizations on the W chromosome were confirmed by fluorescence in-situ hybridization. The 2d-2D9 sequence has no significant homology with other genes in databases but 2d-2F9 has a region which shows partial homology to the consensus sequence of the AAA ATPase superfamily. Both 2d-2D9 and 2d-2F9 sequences are found in contigs of undetermined chromosome-linkage in the Draft Chicken Genome Sequence. |
| Starting Page | 741 |
| Ending Page | 754 |
| Page Count | 14 |
| File Format | |
| ISSN | 09673849 |
| Journal | Chromosome Research |
| Volume Number | 12 |
| Issue Number | 7 |
| e-ISSN | 15736849 |
| Language | English |
| Publisher | Kluwer Academic Publishers |
| Publisher Date | 2004-01-01 |
| Publisher Place | Dordrecht |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Human Genetics Plant Genetics & Genomics Animal Genetics and Genomics |
| Content Type | Text |
| Resource Type | Article |
| Subject | Genetics |
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