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| Content Provider | Springer Nature Link |
|---|---|
| Author | Borowska, Natalia Idziak, Dominika Hasterok, Robert |
| Copyright Year | 2011 |
| Abstract | Sequential immunolocalisation of 5-methylcytosine (5-MeC) and fluorescence in situ hybridisation with chromosome-specific BAC clones were performed on Brachypodium distachyon mitotic metaphase chromosomes to determine specific DNA methylation patterns of each chromosome in the complement. In the majority of cells examined, chromosomes Bd4 and Bd5, which bear the loci of 5S and 35S ribosomal DNA, respectively, had characteristic 5-MeC patterns. In contrast, the distribution of 5-MeC along the metacentric chromosome pairs Bd1, Bd2 and Bd3 was more variable. There were numerous differences in distribution of methylated sites between homologous chromosomes as well as between chromosome arms. Some chromosome sites, such as pericentromeric regions, were highly methylated in all chromosomes. Additionally, the influence of a hypomethylating agent, 5-azacytidine, on B. distachyon chromosome methylation patterns was confirmed. It was found that some chromosome pairs underwent demethylation more easily than others, but there was no apparent regularity in demethylation of particular chromosome segments. |
| Starting Page | 955 |
| Ending Page | 967 |
| Page Count | 13 |
| File Format | |
| ISSN | 09673849 |
| Journal | Chromosome Research |
| Volume Number | 19 |
| Issue Number | 8 |
| e-ISSN | 15736849 |
| Language | English |
| Publisher | Springer Netherlands |
| Publisher Date | 2011-11-11 |
| Publisher Place | Dordrecht |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | 5-azacytidine Brachypodium distachyon Chromosome DNA methylation Epigenetics Cell Biology Animal Genetics and Genomics Plant Genetics & Genomics Human Genetics |
| Content Type | Text |
| Resource Type | Article |
| Subject | Genetics |
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