NDLI: Stability of a Functional Murine Satellite DNA-based Artificial Chromosome Across Mammalian Species

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Stability of a Functional Murine Satellite DNA-based Artificial Chromosome Across Mammalian Species

Content Provider	Springer Nature Link
Author	Telenius, Håkan Szeles, Anna Keresö, Judit Csonka, Erika Prazvszky, Tünde Imreh, Stephan Maxwell, Alexisann Perez, Carl F. Drayer, Jan I. Hadlaczky, Gyula
Copyright Year	1999
Abstract	A 60-Mb murine chromosome consisting of murine pericentric satellite DNA and two bands of integrated marker and reporter genes has been generated de novo in a rodent/human hybrid cell line (mM2C1). This prototype mammalian artificial chromosome platform carries a normal centromere, and the expression of its β-galactosidase reporter gene has remained stable under selection for over 25 months. The novel chromosome was transferred by a modified microcell fusion method to mouse [L-M(TK−)], bovine (P46) and human (EJ30) cell lines. In all cases, the chromosome remained structurally and functionally intact under selection for periods exceeding 3 months from the time of transfer into the new host. In addition, the chromosome was retained in three first- generation tumours when L-M(TK−) cells containing the chromosome were xenografted in severe combined immunodeficiency mice. These data support that a murine satellite DNA-based artificial chromosome can be used as a functional mammalian artificial chromosome and can be maintained in vivo and in cells of heterologous species in vitro.
Starting Page	3
Ending Page	7
Page Count	5
File Format	PDF
ISSN	09673849
Journal	Chromosome Research
Volume Number	7
Issue Number	1
e-ISSN	15736849
Language	English
Publisher	Kluwer Academic Publishers
Publisher Date	1999-01-01
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Human Genetics Plant Genetics & Genomics Animal Genetics and Genomics
Content Type	Text
Resource Type	Article
Subject	Genetics

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