NDLI: Karyotype of canine soft tissue sarcomas: a multi-colour, multi-species approach to canine chromosome painting

Content Provider	Springer Nature Link
Author	Milne, Bruce S. Hoather, Tess O’Brien, Patricia C.M. Yang, Fengtang Ferguson Smith, Malcolm A. Dobson, Jane Sargan, David
Copyright Year	2004
Abstract	Many canine tumour types represent useful models for tumours also found in humans. Studies of chromosomal abnormalities in canine tumours have been impeded by the complexity of the canine karyotype (2n = 78), which has made accurate identification of rearranged chromosomes difficult and laborious. To overcome this difficulty we have developed a seven-colour paint system for canine chromosomes, with six sets of chromosome paints covering all chromosomes except Y. Several pairs of canine autosomes co-locate in the flow karyotype. To distinguish these autosomes from each other, paint sets were supplemented with chromosomes of red fox and Japanese raccoon dog. Paints were used in fluorescence in-situ hybridization to analyse karyotypes in fourteen canine soft tissue sarcomas. Rearranged karyotypes were observed in seven tumours, but there was evidence for loss of rearrangement during tissue culture. Five tumours had rearrangements involving four chromosomes or fewer; one, a chondrosarcoma, had lost seven chromosomes whilst the last, a spindle cell sarcoma, had rearrangements involving eighteen chromosome pairs. The paint sets described here facilitate the complete cytogenetic analysis of balanced translocations and other inter-chromosomal rearrangements in canine tumours. We believe that this is the first canine tumour series to be subjected to this level of analysis.
Starting Page	825
Ending Page	835
Page Count	11
File Format	PDF
ISSN	09673849
Journal	Chromosome Research
Volume Number	12
Issue Number	8
e-ISSN	15736849
Language	English
Publisher	Kluwer Academic Publishers
Publisher Date	2004-01-01
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	canine dog fluorescence in-situ hybridization sarcoma translocations Human Genetics Cell Biology Plant Genetics & Genomics Animal Genetics and Genomics
Content Type	Text
Resource Type	Article
Subject	Genetics

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