NDLI: Integrated cytogenetic BAC map of the genome of the gray, short-tailed opossum, Monodelphis domestica

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Integrated cytogenetic BAC map of the genome of the gray, short-tailed opossum, Monodelphis domestica

Content Provider	Springer Nature Link
Author	Duke, S. E. Samollow, P. B. Mauceli, E. Lindblad Toh, K. Breen, M.
Copyright Year	2007
Abstract	The generation of high-quality genome assemblies for numerous species is advancing at a rapid pace. As the number of genome assemblies increases, so does our ability to investigate genome relationships and their contributions to unraveling complex biological, evolutionary, and biomedical processes. A key process in the generation of a genome assembly is to determine and verify the precise physical location and order of the large sequence blocks (scaffolds) that result from the assembly. For organisms of relatively recent common ancestry this process may be achieved largely through comparative sequence alignment. However, as the evolutionary distance between species lengthens, the use of comparative sequence alignment becomes increasingly less reliable. Simultaneous cytogenetic mapping, using multicolor fluorescence in-situ hybridization (FISH) analysis, offers an alternative means to define the cytogenetic location and relative order of DNA sequences, thereby anchoring the genome sequence to the karyotype. In this article we report the molecular cytogenetic locations of 415 bacterial artificial chromosome (BAC) clones that served to anchor sequence scaffolds of the gray, short-tailed opossum (Monodelphis domestica) to its karyotype, which enabled accurate integration of these regions into the genome assembly.
Starting Page	361
Ending Page	370
Page Count	10
File Format	PDF
ISSN	09673849
Journal	Chromosome Research
Volume Number	15
Issue Number	3
e-ISSN	15736849
Language	English
Publisher	Kluwer Academic Publishers
Publisher Date	2007-05-10
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	chromosome cytogenetic genome map Monodelphis Cell Biology Human Genetics Animal Genetics and Genomics Plant Genetics & Genomics
Content Type	Text
Resource Type	Article
Subject	Genetics

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