NDLI: High-resolution fluorescence in situ hybridization of human Y-linked genes on released chromatin

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High-resolution fluorescence in situ hybridization of human Y-linked genes on released chromatin

Content Provider	Springer Nature Link
Author	Gla¨ser, Birgitta Hierl, Thomas Taylor, Kay Schiebel, Katrin Zeitler, Sylvia Papadopoullos, Katia Rappold, Gudrun Schempp, Werner
Copyright Year	1997
Abstract	Genes within the differential region of the human Y chromosome do not recombine, and therefore the determination of their location depends on physical mapping. Yeast artificial chromosome (YAC) contigs spanning the euchromatic region of the human Y have become a powerful tool for the generation of an overlapping clone map. With this approach,however, complete physical mapping is difficult in Y euchromatic regions that are rich in repetitive sequences. We have, therefore, made use of the fluorescence in situ hybridization technique as an alternative strategy for physically mapping the PRKY and AMELY genes as well as the TSPY, RBM and DAZ gene families to human Y chromosomes in prometaphase and to extended Y chromatin in interphase. From our results, the following order of gene sequences in interval 3 of the short arm of the human Y chromosome is suggested: TSPY major with few RBM sequences interspersed-PRKY-AMELY-TSPY minor with few RBM sequences interspersed-cen. On the long arm, RBM sequences appear to be distributed over wide regions of intervals 5 and 6 with few TSPY sequences interspersed. Distal to an RBM signal cluster, a large cluster of DAZ signals is located with only a few DAZ and RBM signals overlapping in between the two clusters.
Starting Page	23
Ending Page	30
Page Count	8
File Format	PDF
ISSN	09673849
Journal	Chromosome Research
Volume Number	5
Issue Number	1
e-ISSN	15736849
Language	English
Publisher	Kluwer Academic Publishers
Publisher Date	1997-01-01
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Human Genetics Plant Genetics & Genomics Animal Genetics and Genomics
Content Type	Text
Resource Type	Article
Subject	Genetics

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