NDLI: Nuclei of chicken neurons in tissues and three-dimensional cell cultures are organized into distinct radial zones

Content Provider	Springer Nature Link
Author	Berchtold, Doris Fesser, Stephanie Bachmann, Gesine Kaiser, Alexander Eilert, John Christian Frohns, Florian Sadoni, Nicolas Muck, Joscha Kremmer, Elisabeth Eick, Dirk Layer, Paul G. Zink, Daniele
Copyright Year	2011
Abstract	We used chicken retinospheroids (RS) to study the nuclear architecture of vertebrate cells in a three-dimensional (3D) cell culture system. The results showed that the different neuronal cell types of RS displayed an extreme form of radial nuclear organization. Chromatin was arranged into distinct radial zones which became already visible after DAPI staining. The distinct zones were enriched in different chromatin modifications and in different types of chromosomes. Active isoforms of RNA polymerase II were depleted in the outermost zone. Also chromocenters and nucleoli were radially aligned in the nuclear interior. The splicing factor SC35 was enriched at the central zone and did not show the typical speckled pattern of distribution. Evaluation of neuronal and non-neuronal chicken tissues showed that the highly ordered form of radial nuclear organization was also present in neuronal chicken tissues. Furthermore, the data revealed that the neuron-specific nuclear organization was remodeled when cells spread on a flat substrate. Monolayer cultures of a chicken cell line did not show this extreme form of radial organization. Rather, such monolayer cultures displayed features of nuclear organization which have been described before for many different types of monolayer cells. The finding that an extreme form radial nuclear organization, which has not been described before, is present in RS and tissues, but not in cells spread on a flat substrate, suggests that it would be important to complement studies on nuclear architecture performed with monolayer cells by studies on 3D cell culture systems and tissues.
Starting Page	165
Ending Page	182
Page Count	18
File Format	PDF
ISSN	09673849
Journal	Chromosome Research
Volume Number	19
Issue Number	2
e-ISSN	15736849
Language	English
Publisher	Springer Netherlands
Publisher Date	2011-01-20
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	nuclear architecture radial nuclear organization 3D cell culture retinospheroid chicken neuron Cell Biology Animal Genetics and Genomics Human Genetics Plant Genetics & Genomics
Content Type	Text
Resource Type	Article
Subject	Genetics

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Differentiation of embryonic stem cells into fibroblast-like cells in three-dimensional type I collagen gel cultures

Three-dimensional architecture of tandem repeats in chicken interphase nucleus

The radial nuclear positioning of genes correlates with features of megabase-sized chromatin domains

The effects of three-dimensional cell culture on single myoblasts

Bio-stretch, a computerized cell strain apparatus for three-dimensional organotypic cultures

Nuclear architecture of resting and LPS-stimulated porcine neutrophils by 3D FISH

Analysis methods for studying the 3D architecture of the genome

Variable optimization for the formation of three-dimensional self-organized heart muscle

A three-dimensional culture model of canine uterine glands

Nuclei of chicken neurons in tissues and three-dimensional cell cultures are organized into distinct radial zones

Similar Documents

Differentiation of embryonic stem cells into fibroblast-like cells in three-dimensional type I collagen gel cultures

Three-dimensional architecture of tandem repeats in chicken interphase nucleus

The radial nuclear positioning of genes correlates with features of megabase-sized chromatin domains

The effects of three-dimensional cell culture on single myoblasts

Bio-stretch, a computerized cell strain apparatus for three-dimensional organotypic cultures

Nuclear architecture of resting and LPS-stimulated porcine neutrophils by 3D FISH

Analysis methods for studying the 3D architecture of the genome

Variable optimization for the formation of three-dimensional self-organized heart muscle

A three-dimensional culture model of canine uterine glands

Nuclei of chicken neurons in tissues and three-dimensional cell cultures are organized into distinct radial zones