NDLI: Globular and Fibrous Structure in Barley Chromosomes Revealed by High-Resolution Scanning Electron Microscopy

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Globular and Fibrous Structure in Barley Chromosomes Revealed by High-Resolution Scanning Electron Microscopy

Book Reviews : Chromosome Research , Volume 5 , Issue 5

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Chromosome Research : Volume 5, Issue 4, June 1997

Chromosome Research : Volume 5, Issue 3, April 1997

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Chromosome Research : Volume 5, Issue 1, February 1997

Globular and Fibrous Structure in Barley Chromosomes Revealed by High-Resolution Scanning Electron Microscopy

Content Provider	Springer Nature Link
Author	Iwa, Megumi Fukui, Kiichi Takaichi, Shigeko Isogai, Akira
Copyright Year	1997
Abstract	Barley chromosomes were prepared for high-resolution scanning electron microscopy using a combination of enzyme maceration, treatment in acetic acid and osmium impregnation using thiocarbohydrazide. Using this technique, the three-dimensional ultra-structure of interphase nuclei and mitotic chromosomes was examined. In interphase, different levels of chromatin condensation were observed, consisting of fibrils 10 nm in diameter, 20- to 40-nm fibres and a higher order complex. In prophase, globular and strand-like structures composed of 20- to 40-nm fibres were dominant. As the cells progressed through the cell cycle and the chromatin condensed, globular and strand-like structures (chromomeres) were coiled and packed to form chromosomes. Chromomeres were observed as globular protuberances on the surface of metaphase chromosomes. These findings indicate that the chromomere is a fundamental substructure of the higher order architecture of the chromosome. In the centromeric region, there were no globular protuberances, but 20- to 40-nm fibres were folded compactly to form a higher level organization surrounding the chromosomal axis.
Starting Page	341
Ending Page	349
Page Count	9
File Format	PDF
ISSN	09673849
Journal	Chromosome Research
Volume Number	5
Issue Number	5
e-ISSN	15736849
Language	English
Publisher	Kluwer Academic Publishers
Publisher Date	1997-01-01
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Human Genetics Cell Biology Plant Genetics & Genomics Animal Genetics and Genomics
Content Type	Text
Resource Type	Article
Subject	Genetics

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