NDLI: Differential PARP Cleavage: An Indication of Heterogeneous Forms of Cell Death and Involvement of Multiple Proteases in the Infarct of Focal Cerebral Ischemia in Rat

Please wait, while we are loading the content...

Rutin Supplementation in the Diet has Protective Effects Against Toxicant-Induced Hippocampal Injury by Suppression of Microglial Activation and Pro-Inflammatory Cytokines : Protective Effect of Rutin Against Toxicant-Induced Hippocampal Injury

Transcriptome Profiling of Neuronal Model Cell PC12 from Rat Pheochromocytoma

Fibronectin and Focal Adhesion Kinase Small Interfering RNA Modulate Rat Retinal Müller Cells Adhesion and Migration

Quantitative Analysis of Delayed Neuronal Death in the Hippocampal Subfields of SHRSP and SHR

Differential PARP Cleavage: An Indication of Heterogeneous Forms of Cell Death and Involvement of Multiple Proteases in the Infarct of Focal Cerebral Ischemia in Rat

Synaptic Degeneration of Retinal Ganglion Cells in a Rat Ocular Hypertension Glaucoma Model

Expression of Corticosteroid-Binding Globulin in Human Astrocytoma Cell Line

Protection of PMS777, a New AChE Inhibitor with PAF Antagonism, Against Amyloid-β-Induced Neuronal Apoptosis and Neuroinflammation

Expression of the Dominant-Negative Tail of Myosin Va Enhances Exocytosis of Large Dense Core Vesicles in Neurons

Cellular and Molecular Neurobiology : Volume 29, Issue 3, May 2009

Cellular and Molecular Neurobiology : Volume 29, Issue 2, March 2009

Cellular and Molecular Neurobiology : Volume 29, Issue 1, February 2009

Cellular and Molecular Neurobiology : Volume 28

Cellular and Molecular Neurobiology : Volume 27

Cellular and Molecular Neurobiology : Volume 26

Cellular and Molecular Neurobiology : Volume 25

Cellular and Molecular Neurobiology : Volume 24

Cellular and Molecular Neurobiology : Volume 23

Cellular and Molecular Neurobiology : Volume 22

Cellular and Molecular Neurobiology : Volume 21

Cellular and Molecular Neurobiology : Volume 20

Cellular and Molecular Neurobiology : Volume 19

Cellular and Molecular Neurobiology : Volume 18

Cellular and Molecular Neurobiology : Volume 17

Differential PARP Cleavage: An Indication of Heterogeneous Forms of Cell Death and Involvement of Multiple Proteases in the Infarct of Focal Cerebral Ischemia in Rat

Content Provider	Springer Nature Link
Author	Chaitanya, Ganta Vijay Babu, Phanithi Prakash
Copyright Year	2009
Abstract	Aim Poly (ADP-ribose) polymerase (PARP) is a nuclear repair enzyme whose role is widely depicted in various physiological and pathological processes. In the present study, we wanted to check the status of PARP and the role of various cell death proteases involved in apoptotic and non-apoptotic forms of cell death during transient focal cerebral ischemia in rat model. The activation of these proteases can result in the production of PARP fragments which can be treated as specific signature fragments to the particular protease involved in the pathology and hence the type of cell death. Results In the ischemic samples, we observed activation of calpain, cathepsin-b, caspase-3, and granzyme-b which were known to act on and cleave PARP to produce specific signature fragments by Western blot and immunohistochemical analysis. Cresyl violet staining showed the presence of apoptotic and necrotic cell deaths. Further we observed interaction of AIF and gra-b with PARP in double immunofluorescence and co-immunoprecipitation experiments. Conclusion Activation of calpains, cathepsin-b, caspase-3, and granzyme-b correlated with either apoptotic or necrotic cell deaths in cresyl violet staining. The appearance of PARP signature fragments gives a clear idea on the involvement of particular protease in the pathology. Appearance of signature fragments like 89- and 50-kDa indicates the involvement of apoptotic and necrotic cell death in the pathology. Further interaction of AIF and gra-b with PARP also indicates the involvement of non-apoptotic modes of cell death during the pathology of focal cerebral ischemia.
Starting Page	563
Ending Page	573
Page Count	11
File Format	PDF
ISSN	02724340
Journal	Cellular and Molecular Neurobiology
Volume Number	29
Issue Number	4
e-ISSN	15736830
Language	English
Publisher	Springer US
Publisher Date	2009-02-19
Publisher Place	Boston
Access Restriction	Subscribed
Subject Keyword	Cerebral ischemia PARP Granzyme-b AIF Apoptosis Necrosis Animal Anatomy / Morphology / Histology Neurosciences
Content Type	Text
Resource Type	Article
Subject	Cell Biology Medicine Cellular and Molecular Neuroscience

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Neuroprotective Mechanism of Taurine due to Up-regulating Calpastatin and Down-regulating Calpain and Caspase-3 during Focal Cerebral Ischemia

Involvement of Glial Endothelin/Nitric Oxide in Delayed Neuronal Death of Rat Hippocampus After Transient Forebrain Ischemia

Mechanisms of lysosomal proteases participating in cerebral ischemia-induced neuronal death

Cross-talk Between Calpain and Caspase-3 in Penumbra and Core During Focal Cerebral Ischemia-reperfusion

Apelin-13 Protects the Brain Against Ischemic Reperfusion Injury and Cerebral Edema in a Transient Model of Focal Cerebral Ischemia

Dynamic Alterations of Cerebral Pial Microcirculation During Experimental Subarachnoid Hemorrhage

Oxaloacetate Decreases the Infarct Size and Attenuates the Reduction in Evoked Responses after Photothrombotic Focal Ischemia in the Rat Cortex

Mitochondria, oxidative metabolism and cell death in stroke

Kainate-Mediated Excitotoxicity Induces Neuronal Death in the Rat Spinal Cord In Vitro via a PARP-1 Dependent Cell Death Pathway (Parthanatos)

Differential PARP Cleavage: An Indication of Heterogeneous Forms of Cell Death and Involvement of Multiple Proteases in the Infarct of Focal Cerebral Ischemia in Rat

Similar Documents

Neuroprotective Mechanism of Taurine due to Up-regulating Calpastatin and Down-regulating Calpain and Caspase-3 during Focal Cerebral Ischemia

Involvement of Glial Endothelin/Nitric Oxide in Delayed Neuronal Death of Rat Hippocampus After Transient Forebrain Ischemia

Mechanisms of lysosomal proteases participating in cerebral ischemia-induced neuronal death

Cross-talk Between Calpain and Caspase-3 in Penumbra and Core During Focal Cerebral Ischemia-reperfusion

Apelin-13 Protects the Brain Against Ischemic Reperfusion Injury and Cerebral Edema in a Transient Model of Focal Cerebral Ischemia

Dynamic Alterations of Cerebral Pial Microcirculation During Experimental Subarachnoid Hemorrhage

Oxaloacetate Decreases the Infarct Size and Attenuates the Reduction in Evoked Responses after Photothrombotic Focal Ischemia in the Rat Cortex

Mitochondria, oxidative metabolism and cell death in stroke

Kainate-Mediated Excitotoxicity Induces Neuronal Death in the Rat Spinal Cord In Vitro via a PARP-1 Dependent Cell Death Pathway (Parthanatos)

Differential PARP Cleavage: An Indication of Heterogeneous Forms of Cell Death and Involvement of Multiple Proteases in the Infarct of Focal Cerebral Ischemia in Rat