NDLI: Repression of SIRT1 Promotes the Differentiation of Mouse Induced Pluripotent Stem Cells into Neural Stem Cells

Content Provider	Springer Nature Link
Author	Hu, Bin Guo, Ye Chen, Chunyuan Li, Qing Niu, Xin Guo, Shangchun Zhang, Aijun Wang, Yang Deng, Zhifeng
Copyright Year	2014
Abstract	The use of transplanting functional neural stem cells (NSCs) derived from induced pluripotent stem cells (iPSCs) has increased for the treatment of brain diseases. As such, it is important to understand the molecular mechanisms that promote NSCs differentiation of iPSCs for future NSC-based therapies. Sirtuin 1 (SIRT1), a NAD+-dependent protein deacetylase, has attracted significant attention over the past decade due to its prominent role in processes including organ development, longevity, and cancer. However, it remains unclear whether SIRT1 plays a role in the differentiation of mouse iPSCs toward NSCs. In this study, we produced NSCs from mouse iPSCs using serum-free medium supplemented with retinoic acid. We then assessed changes in the expression of SIRT1 and microRNA-34a, which regulates SIRT1 expression. Moreover, we used a SIRT1 inhibitor to investigate the role of SIRT1 in NSCs differentiation of iPSCs. Data revealed that the expression of SIRT1 decreased, whereas miRNAs-34a increased, during this process. In addition, the inhibition of SIRT1 enhanced the generation of NSCs and mature neurocytes. This suggests that SIRT1 negatively regulated the differentiation of mouse iPSCs into NSCs, and that this process may be regulated by miRNA-34a.
Starting Page	905
Ending Page	912
Page Count	8
File Format	PDF
ISSN	02724340
Journal	Cellular and Molecular Neurobiology
Volume Number	34
Issue Number	6
e-ISSN	15736830
Language	English
Publisher	Springer US
Publisher Date	2014-05-15
Publisher Place	Boston
Access Restriction	Subscribed
Subject Keyword	Induced pluripotent stem cell Neural stem cell Differentiation SIRT1 microRNA-34a Neurosciences Cell Biology Neurobiology
Content Type	Text
Resource Type	Article
Subject	Cell Biology Medicine Cellular and Molecular Neuroscience

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