NDLI: A Trifluoromethyl Analog of Verbenachalcone Promotes Neurite Outgrowth and Cell Proliferation of NeuroScreen-1 Cells

Please wait, while we are loading the content...

Characterizing Photothrombotic Distal Middle Cerebral Artery Occlusion and YAG Laser-Induced Reperfusion Model in the Izumo Strain of Spontaneously Hypertensive Rats

Induction of Apoptosis by Melamine in Differentiated PC12 Cells

Neuroprotective Effects of Active Ingredients Isolated from Pegasus laternarius on Cultured Cerebral Neurons

Beneficial Effects of Folic Acid on Enhancement of Memory and Antioxidant Status in Aged Rat Brain

Transient Expression of Iron Transport Proteins in the Capillary of the Developing Rat Brain

The Role of β-Catenin Signaling Pathway on Proliferation of Rats Neural Stem Cells After Hyperbaric Oxygen Therapy In Vitro

Reversal of Rotenone-Induced Dysfunction of Astrocytic Connexin43 by Opening Mitochondrial ATP-Sensitive Potassium Channels

Continuous Expression of HIF-1α in Neural Stem/Progenitor Cells

Downregulation of the cAMP/PKA Pathway in PC12 Cells Overexpressing NCS-1

A Trifluoromethyl Analog of Verbenachalcone Promotes Neurite Outgrowth and Cell Proliferation of NeuroScreen-1 Cells

Increase of CGRP Expression in Motor Endplates Within Fore and Hind Limb Muscles of the Degenerating Muscle Mouse (Scn8a dmu)

The Wnt Signaling Pathway Protects Retinal Ganglion Cell 5 (RGC-5) Cells from Elevated Pressure

Cellular and Molecular Neurobiology : Volume 30

Cellular and Molecular Neurobiology : Volume 29

Cellular and Molecular Neurobiology : Volume 28

Cellular and Molecular Neurobiology : Volume 27

Cellular and Molecular Neurobiology : Volume 26

Cellular and Molecular Neurobiology : Volume 25

Cellular and Molecular Neurobiology : Volume 24

Cellular and Molecular Neurobiology : Volume 23

Cellular and Molecular Neurobiology : Volume 22

Cellular and Molecular Neurobiology : Volume 21

Cellular and Molecular Neurobiology : Volume 20

Cellular and Molecular Neurobiology : Volume 19

Cellular and Molecular Neurobiology : Volume 18

Cellular and Molecular Neurobiology : Volume 17

A Trifluoromethyl Analog of Verbenachalcone Promotes Neurite Outgrowth and Cell Proliferation of NeuroScreen-1 Cells

Content Provider	Springer Nature Link
Author	Yeyeodu, Susan Gilyazova, Nailya Huh, Eun Young Dandepally, Srinivasa R. Oldham, Carla Williams, Alfred Ibeanu, Gordon
Copyright Year	2010
Abstract	Past research has shown that natural products of plant and marine origins and their congeners enhance the actions of neuritogenic factors of the central nervous system (CNS) such as nerve growth factor (NGF). However, the role of fluorine substitutions in their structure–activity relationship (SAR) has not been explored. We have synthesized a trifluoromethyl analog of verbenachalcone (VC), a pharmacologically active natural compound previously shown to potentiate NGF activity. This analog, designated C278, enhances neurite outgrowth and proliferation of NeuroScreen-1™ (NS-1) cells, a subclone of PC12 pheochromocytoma cells. C278 increases the percentage of neurite bearing cells in the presence of suboptimal doses of NGF in comparison with controls treated with NGF alone. In addition, C278 stimulates cell growth in reduced serum and serum-free cell culture conditions based on our observation of increases in cell number and metabolic assessment with MTT reduction and resazurin assays. The addition of C278 partially restored inhibition of NGF-induced neurite outgrowth by the mitogen-activated protein kinase kinase (MEK) inhibitors PD98059 and U0126. Short-term sequential exposure of cells to U0126, C278, and NGF enhanced phosphorylation of extracellular signal-regulated kinase (ERK) in comparison with cells treated with only the MEK inhibitor and NGF. C278 also attenuated cell growth arrest caused by exposure to PD98059, U0126 and the phosphatidylinositol-3 kinase (PI3K) inhibitor, LY294002 but did not alter phosphorylation of Akt, a classic downstream target of PI3K during cell survival. These data suggest that C278 promotes NGF-dependent neurite outgrowth in NS-1 cells through a MEK signaling pathway by a mechanism that alters short-term activation of ERK. In contrast, C278 promotes PI3K-mediated survival independently of Akt phosphorylation.
Starting Page	145
Ending Page	153
Page Count	9
File Format	PDF
ISSN	02724340
Journal	Cellular and Molecular Neurobiology
Volume Number	31
Issue Number	1
e-ISSN	15736830
Language	English
Publisher	Springer US
Publisher Date	2010-09-17
Publisher Place	Boston
Access Restriction	Subscribed
Subject Keyword	Neurite outgrowth Potentiator NGF Verbenachalcone Trifluoromethyl analog NeuroScreen-1 Neuroprotective NS-1 PC12 Natural product Neurobiology Cell Biology Neurosciences
Content Type	Text
Resource Type	Article
Subject	Cell Biology Medicine Cellular and Molecular Neuroscience

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

c-Jun N-terminal kinase in synergistic neurite outgrowth in PC12 cells mediated through P90RSK

Minocycline Promotes Neurite Outgrowth of PC12 Cells Exposed to Oxygen-Glucose Deprivation and Reoxygenation Through Regulation of MLCP/MLC Signaling Pathways

Transcription Factor Brn-3b Overexpression Enhances Neurite Outgrowth in PC12 Cells Under Condition of Hypoxia

MicroRNA-29c/PTEN Pathway is Involved in Mice Brain Development and Modulates Neurite Outgrowth in PC12 Cells

Prenatal cerebrospinal fluid modulate differentiation and proliferation of rat pheochoromocytoma PC12 cells

Liraglutide Promotes Cortical Neurite Outgrowth via the MEK–ERK Pathway

Non-cytotoxic Concentration of Cisplatin Decreases Neuroplasticity-Related Proteins and Neurite Outgrowth Without Affecting the Expression of NGF in PC12 Cells

Guanosine stimulates neurite outgrowth in PC12 cells via activation of heme oxygenase and cyclic GMP

Transcriptional Down-regulation of Epidermal Growth Factor (EGF) Receptors by Nerve Growth Factor (NGF) in PC12 Cells

A Trifluoromethyl Analog of Verbenachalcone Promotes Neurite Outgrowth and Cell Proliferation of NeuroScreen-1 Cells

Similar Documents

c-Jun N-terminal kinase in synergistic neurite outgrowth in PC12 cells mediated through P90RSK

Minocycline Promotes Neurite Outgrowth of PC12 Cells Exposed to Oxygen-Glucose Deprivation and Reoxygenation Through Regulation of MLCP/MLC Signaling Pathways

Transcription Factor Brn-3b Overexpression Enhances Neurite Outgrowth in PC12 Cells Under Condition of Hypoxia

MicroRNA-29c/PTEN Pathway is Involved in Mice Brain Development and Modulates Neurite Outgrowth in PC12 Cells

Prenatal cerebrospinal fluid modulate differentiation and proliferation of rat pheochoromocytoma PC12 cells

Liraglutide Promotes Cortical Neurite Outgrowth via the MEK–ERK Pathway

Non-cytotoxic Concentration of Cisplatin Decreases Neuroplasticity-Related Proteins and Neurite Outgrowth Without Affecting the Expression of NGF in PC12 Cells

Guanosine stimulates neurite outgrowth in PC12 cells via activation of heme oxygenase and cyclic GMP

Transcriptional Down-regulation of Epidermal Growth Factor (EGF) Receptors by Nerve Growth Factor (NGF) in PC12 Cells

A Trifluoromethyl Analog of Verbenachalcone Promotes Neurite Outgrowth and Cell Proliferation of NeuroScreen-1 Cells