NDLI: Overexpressed Alpha-Synuclein Regulated the Nuclear Factor-kappaB Signal Pathway

Content Provider	Springer Nature Link
Author	Yuan, Yuhe Jin, Jin Yang, Bo Zhang, Wei Hu, Jinfeng Zhang, Yun Chen, Nai Hong
Copyright Year	2007
Abstract	Alpha-synuclein is a presynaptic protein which is implicated in some neurodegenerative disorders including Parkinson’s disease, dementia with Lewy bodies, multiple systems atrophy, and Hallervorden-Spatz disease, and its overexpression contributes to the loss of dopaminergic neurons. Although the role of alpha-synuclein in these paradigms has been widely documented, its exact function is still elusive. And the dysfunction of the transcription factor nuclear factor (NF-κB) also exists in many neurodegenerative diseases. In this reason the purpose of this study was to investigate the molecular mechanism of alpha-synuclein’s toxicity and its association with NF-κB by MTT assay, Western blot method, and luciferase assay. Results showed that overexpressed alpha-synuclein and 1-methyl-4-phenylpyridinium (MPP+) suppressed the SH-SY5Y cell viability and attenuate NF-κB-mediated luciferase expression significantly. Moreover, the impairment function was enhanced with the increase of alpha-synuclein protein level. We also found that overexpressed alpha-synuclein localized both in the cytoplasms and nuclei, down-regulated the anti-apoptotic Bcl-2 expression and up-regulated the pro-apoptotic glycogen synthase kinase 3β (GSK3β) protein level. In conclusion, all these findings mentioned above suggested that alpha-synuclein shared some toxic functional homology with neurotoxin MPP+, and the proapoptotic effects of alpha-synuclein might be mediated at least in part by the impairment of NF-κB signaling pathway which involves GSK3β.
Starting Page	21
Ending Page	33
Page Count	13
File Format	PDF
ISSN	02724340
Journal	Cellular and Molecular Neurobiology
Volume Number	28
Issue Number	1
e-ISSN	15736830
Language	English
Publisher	Springer US
Publisher Date	2007-08-22
Publisher Place	Boston
Access Restriction	Subscribed
Subject Keyword	Alpha-synuclein NF-κB MPP+ GSK3β Bcl-2 Animal Anatomy / Morphology / Histology Neurosciences
Content Type	Text
Resource Type	Article
Subject	Cell Biology Medicine Cellular and Molecular Neuroscience

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