NDLI: Thyroid Hormone Mediates Syndecan Expression in Rat Neonatal Cerebellum

Content Provider	Springer Nature Link
Author	Mendes de Aguiar, Cláudia Beatriz Nedel Costa Silva, Bru Alvarez Silva, Marcio Tasca, Carla Inês Trentin, Andréa Gonçalves
Copyright Year	2008
Abstract	Thyroid hormone (T3) plays an essential role in the central nervous system development. Astrocytes mediate many of the T3 effects in the growth and differentiation of cerebellum. In culture, T3 induces cerebellar astrocytes to secrete growth factors, mainly FGF2, and alters the expression and organization of the extracellular matrix (ECM) proteins, laminin, and fibronectin. In addition, T3-treated astrocytes promote neuronal differentiation. In this study, we have investigated whether other ECM molecules, such as syndecans, are involved in T3 action. Thus, we analyzed the expression of syndecans (1–4) by RT-PCR in astrocyte cultures from cerebellum, cortex, and hippocampus of newborn rats. Our results showed that syndecans (1–4) are expressed in astrocytes of cerebellum and cortex, whereas in hippocampus only syndecans 2 and 4 were detected. Semi-quantitative RT-PCR analysis revealed the reduced expression of syndecans 1, 2, and 4, and increased expression of syndecan 3 in hypothyroid cerebellum, when compared to the euthyroid tissue. Furthermore, we observed a reduced expression of syndecans 2 and 3 in T3-treated cerebellar astrocytes, when compared to control cultures. This balance of proteoglycans may be involved in T3 action mediated by FGF2 signaling, possibly affecting the formation of the trimeric signaling receptor complex composed by syndecan/FGF/FGF-receptor (FGFR), which is essential for FGFR dimerization, activation, and subsequent cell signaling.
Starting Page	795
Ending Page	801
Page Count	7
File Format	PDF
ISSN	02724340
Journal	Cellular and Molecular Neurobiology
Volume Number	28
Issue Number	6
e-ISSN	15736830
Language	English
Publisher	Springer US
Publisher Date	2008-01-25
Publisher Place	Boston
Access Restriction	Subscribed
Subject Keyword	Syndecan Thyroid hormone Brain Astrocyte Animal Anatomy / Morphology / Histology Neurosciences
Content Type	Text
Resource Type	Article
Subject	Cell Biology Medicine Cellular and Molecular Neuroscience

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in