NDLI: Metabolic Characterization of a Mouse Deficient in All Known Leptin Receptor Isoforms

Content Provider	Springer Nature Link
Author	Osborn, Olivia Sanchez Alavez, Manuel Brownell, Sara E. Ross, Brendon Klaus, Joe Dubins, Jeffrey Beutler, Bruce Conti, Bru Bartfai, Tamas
Copyright Year	2009
Abstract	We have characterized a newly generated mouse model of obesity, a mouse strain deficient in all five previously described leptin receptor isoforms. These transgenic mice, named the db 333/db 333 mice, were identified from an ENU mutagenesis screen and carry a point mutation in the seventh exon of the db gene encoding the leptin receptor, resulting in a premature stop codon (Y333Stop) and gene product that lacks STAT signaling domains. db 333/db 333 mice have a morbidly obese phenotype, with body weights diverging from wild type as early as 4 weeks of age (P < 0.05). To determine the contribution of the short isoforms of the leptin receptor in this metabolic phenotype, we performed an extensive metabolic characterization of the db 333/db 333 mouse in relation to the well-characterized db/db mouse lacking only the long form of the leptin receptor. db 333/db 333 mice have similar endocrine and metabolic parameters as previously described in other leptin receptor transgenic mice including db/db mice that lack only the long isoform of the leptin receptor. However, db 333/db 333 mice show a subtle trend toward higher body weight and insulin levels, lower oxygen, carbon dioxide production, respiratory exchange ratio (RER), and temperature than db/db mice suggesting the short isoforms may play an additional role in energy homeostasis.
Starting Page	23
Ending Page	33
Page Count	11
File Format	PDF
ISSN	02724340
Journal	Cellular and Molecular Neurobiology
Volume Number	30
Issue Number	1
e-ISSN	15736830
Language	English
Publisher	Springer US
Publisher Date	2009-07-07
Publisher Place	Boston
Access Restriction	Subscribed
Subject Keyword	Leptin Leptin receptor Ob–Rb Obesity db/db db333/db333 Diabetes Insulin resistance Animal Anatomy / Morphology / Histology Neurosciences
Content Type	Text
Resource Type	Article
Subject	Cell Biology Medicine Cellular and Molecular Neuroscience

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